Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.

2.50
Hdl Handle:
http://hdl.handle.net/10033/270921
Title:
Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.
Authors:
Kaufmann, Katrin; Simmons, Luke; Herrmann, Jennifer; Schwär, Gertrud; Luniak, Nora; Müller, Rolf
Abstract:
Using an in vitro cell-based assay in a flow-design, we have applied activity-guided screening to search for new bioactive compounds isolated from microorganisms. A first assay employs the stable expression of nuclear factor kappa B (NF-κB) while a second assay utilizes the glucocorticoid receptor (GR) coupled to green fluorescent protein. A specialized assay was implemented for both the translocation of NF-κB and to inhibit the translocation of cytokine-mediated NF-κB. In addition, we developed in a wide palette of cell lines used for a highly specialized GR-translocation assay to detect anti-inflammatory effects. This approach demonstrates the straight-forward combination of cell-based assays arranged with an automated fluorescence microscope. This allows for the direct sorting of extracts which are acting in a pharmaceutically interesting way. Initial results using this technique have led to the detection of new anti-inflammatory steroids from bacterial crude extracts.
Affiliation:
Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken, Germany. kaufm@uni-trier.de
Citation:
Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria. 2013, 35 (1):11-20 Biotechnol. Lett.
Journal:
Biotechnology letters
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10033/270921
DOI:
10.1007/s10529-012-1042-0
PubMed ID:
22983717
Type:
Article
Language:
en
ISSN:
1573-6776
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorKaufmann, Katrinen_GB
dc.contributor.authorSimmons, Lukeen_GB
dc.contributor.authorHerrmann, Jenniferen_GB
dc.contributor.authorSchwär, Gertruden_GB
dc.contributor.authorLuniak, Noraen_GB
dc.contributor.authorMüller, Rolfen_GB
dc.date.accessioned2013-03-04T13:38:31Z-
dc.date.available2013-03-04T13:38:31Z-
dc.date.issued2013-01-
dc.identifier.citationActivity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria. 2013, 35 (1):11-20 Biotechnol. Lett.en_GB
dc.identifier.issn1573-6776-
dc.identifier.pmid22983717-
dc.identifier.doi10.1007/s10529-012-1042-0-
dc.identifier.urihttp://hdl.handle.net/10033/270921-
dc.description.abstractUsing an in vitro cell-based assay in a flow-design, we have applied activity-guided screening to search for new bioactive compounds isolated from microorganisms. A first assay employs the stable expression of nuclear factor kappa B (NF-κB) while a second assay utilizes the glucocorticoid receptor (GR) coupled to green fluorescent protein. A specialized assay was implemented for both the translocation of NF-κB and to inhibit the translocation of cytokine-mediated NF-κB. In addition, we developed in a wide palette of cell lines used for a highly specialized GR-translocation assay to detect anti-inflammatory effects. This approach demonstrates the straight-forward combination of cell-based assays arranged with an automated fluorescence microscope. This allows for the direct sorting of extracts which are acting in a pharmaceutically interesting way. Initial results using this technique have led to the detection of new anti-inflammatory steroids from bacterial crude extracts.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Biotechnology lettersen_GB
dc.titleActivity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pharmaceutical Biotechnology, Saarland University, Saarbrücken, Germany. kaufm@uni-trier.deen_GB
dc.identifier.journalBiotechnology lettersen_GB

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