Pseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate.

2.50
Hdl Handle:
http://hdl.handle.net/10033/27612
Title:
Pseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate.
Authors:
Meissner, Andree; Wild, Verena; Simm, Roger; Rohde, Manfred; Erck, Christian; Bredenbruch, Florian; Morr, Michael; Römling, Ute; Häussler, Susanne
Abstract:
Cyclic-diguanylate (c-di-GMP) is a widespread bacterial signal molecule that plays a major role in the modulation of cellular surface components, such as exopolysaccharides and fimbriae, and in the establishment of a sessile life style. Here, we report that intracellular c-di-GMP levels influence cupA-encoded fimbriae expression in Pseudomonas aeruginosa. In an autoaggregative P. aeruginosa small colony variant (SCV) CupA fimbriae and the intracellular c-di-GMP concentration were found to be enhanced as compared with the clonal wild-type. The SCV morphology and the expression of CupA fimbriae were dependent on a functional PA1120 and morA gene both encoding a GGDEF domain. Overexpression of the GGDEF domain protein PA1120 complemented the PA1120 and the morA mutant with respect to CupA fimbriae expression. In agreement with these findings, overexpression of the EAL domain containing phenotypic variance regulator (PvrR) in the SCV resulted in a decreased intracellular level of c-di-GMP, a reduced cupA fimbriae expression and a switch to wild-type colony morphology.
Affiliation:
Division of Cell Biology and Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Citation:
Pseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate. 2007, 9 (10):2475-85 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Oct-2007
URI:
http://hdl.handle.net/10033/27612
DOI:
10.1111/j.1462-2920.2007.01366.x
PubMed ID:
17803773
Type:
Article
Language:
en
ISSN:
1462-2912
Appears in Collections:
publications of the research group chronic pseudomonas infections (CPI)

Full metadata record

DC FieldValue Language
dc.contributor.authorMeissner, Andree-
dc.contributor.authorWild, Verena-
dc.contributor.authorSimm, Roger-
dc.contributor.authorRohde, Manfred-
dc.contributor.authorErck, Christian-
dc.contributor.authorBredenbruch, Florian-
dc.contributor.authorMorr, Michael-
dc.contributor.authorRömling, Ute-
dc.contributor.authorHäussler, Susanne-
dc.date.accessioned2008-05-22T12:45:59Z-
dc.date.available2008-05-22T12:45:59Z-
dc.date.issued2007-10-
dc.identifier.citationPseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate. 2007, 9 (10):2475-85 Environ. Microbiol.en
dc.identifier.issn1462-2912-
dc.identifier.pmid17803773-
dc.identifier.doi10.1111/j.1462-2920.2007.01366.x-
dc.identifier.urihttp://hdl.handle.net/10033/27612-
dc.description.abstractCyclic-diguanylate (c-di-GMP) is a widespread bacterial signal molecule that plays a major role in the modulation of cellular surface components, such as exopolysaccharides and fimbriae, and in the establishment of a sessile life style. Here, we report that intracellular c-di-GMP levels influence cupA-encoded fimbriae expression in Pseudomonas aeruginosa. In an autoaggregative P. aeruginosa small colony variant (SCV) CupA fimbriae and the intracellular c-di-GMP concentration were found to be enhanced as compared with the clonal wild-type. The SCV morphology and the expression of CupA fimbriae were dependent on a functional PA1120 and morA gene both encoding a GGDEF domain. Overexpression of the GGDEF domain protein PA1120 complemented the PA1120 and the morA mutant with respect to CupA fimbriae expression. In agreement with these findings, overexpression of the EAL domain containing phenotypic variance regulator (PvrR) in the SCV resulted in a decreased intracellular level of c-di-GMP, a reduced cupA fimbriae expression and a switch to wild-type colony morphology.en
dc.language.isoenen
dc.subject.meshCyclic GMPen
dc.subject.meshFimbriae Proteinsen
dc.subject.meshFimbriae, Bacterialen
dc.subject.meshHumansen
dc.subject.meshPhenotypeen
dc.subject.meshProtein Structure, Tertiaryen
dc.subject.meshPseudomonas aeruginosaen
dc.titlePseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate.en
dc.typeArticleen
dc.contributor.departmentDivision of Cell Biology and Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.en
dc.identifier.journalEnvironmental microbiologyen

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