2.50
Hdl Handle:
http://hdl.handle.net/10033/276164
Title:
Growth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus.
Authors:
Takacs, Constantin N; Hocking, Jason; Cabeen, Matthew T; Bui, Nhat Khai; Poggio, Sebastian; Vollmer, Waldemar; Jacobs-Wagner, Christine
Abstract:
The peptidoglycan (PG) is a macromolecular component of the bacterial cell wall that maintains the shape and integrity of the cell. The PG of , unlike that of many other Gram-negative bacteria, has repeatedly been shown to contain significant amounts of glycine. This compositional peculiarity has been deemed an intrinsic characteristic of this species. By performing a comprehensive qualitative and quantitative analysis of the PG by high-performance liquid chromatography (HPLC) and mass spectrometry (MS), we show here that glycine incorporation into the PG depends on the presence of exogenous glycine in the growth medium. High levels of glycine were detected at the fifth position of the peptide side chains of PG isolated from cells grown in the complex laboratory medium PYE or in defined medium (M2G) supplemented with casamino acids or glycine alone. In contrast, glycine incorporation was undetectable when cells were grown in M2G medium lacking glycine. Remarkably, glycine incorporation into peptidoglycan occurred even in the presence of low millimolar to sub-millimolar concentrations of free glycine. High glycine content in the PG had no obvious effects on growth rates, mode of PG incorporation or cell morphology. Hence, the PG is able to retain its physiological functions in cell growth and morphogenesis despite significant alterations in its composition, in what we deem to be unprecedented plasticity.
Affiliation:
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America.
Citation:
Growth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus. 2013, 8 (2):e57579 PLoS ONE
Journal:
PloS one
Issue Date:
2013
URI:
http://hdl.handle.net/10033/276164
DOI:
10.1371/journal.pone.0057579
PubMed ID:
23469030
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the department of microbial natural substances ([HIPS]MINS)

Full metadata record

DC FieldValue Language
dc.contributor.authorTakacs, Constantin Nen_GB
dc.contributor.authorHocking, Jasonen_GB
dc.contributor.authorCabeen, Matthew Ten_GB
dc.contributor.authorBui, Nhat Khaien_GB
dc.contributor.authorPoggio, Sebastianen_GB
dc.contributor.authorVollmer, Waldemaren_GB
dc.contributor.authorJacobs-Wagner, Christineen_GB
dc.date.accessioned2013-03-27T13:53:38Z-
dc.date.available2013-03-27T13:53:38Z-
dc.date.issued2013-
dc.identifier.citationGrowth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus. 2013, 8 (2):e57579 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid23469030-
dc.identifier.doi10.1371/journal.pone.0057579-
dc.identifier.urihttp://hdl.handle.net/10033/276164-
dc.description.abstractThe peptidoglycan (PG) is a macromolecular component of the bacterial cell wall that maintains the shape and integrity of the cell. The PG of , unlike that of many other Gram-negative bacteria, has repeatedly been shown to contain significant amounts of glycine. This compositional peculiarity has been deemed an intrinsic characteristic of this species. By performing a comprehensive qualitative and quantitative analysis of the PG by high-performance liquid chromatography (HPLC) and mass spectrometry (MS), we show here that glycine incorporation into the PG depends on the presence of exogenous glycine in the growth medium. High levels of glycine were detected at the fifth position of the peptide side chains of PG isolated from cells grown in the complex laboratory medium PYE or in defined medium (M2G) supplemented with casamino acids or glycine alone. In contrast, glycine incorporation was undetectable when cells were grown in M2G medium lacking glycine. Remarkably, glycine incorporation into peptidoglycan occurred even in the presence of low millimolar to sub-millimolar concentrations of free glycine. High glycine content in the PG had no obvious effects on growth rates, mode of PG incorporation or cell morphology. Hence, the PG is able to retain its physiological functions in cell growth and morphogenesis despite significant alterations in its composition, in what we deem to be unprecedented plasticity.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleGrowth Medium-Dependent Glycine Incorporation into the Peptidoglycan of Caulobacter crescentus.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America.en_GB
dc.identifier.journalPloS oneen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.