Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background.

2.50
Hdl Handle:
http://hdl.handle.net/10033/28152
Title:
Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background.
Authors:
Bleich, Andre; Sundberg, John P; Smoczek, Anna; von Wasielewski, Reinhard; de Buhr, Maike F; Janus, Lydia M; Julga, Gwen; Ukena, Sya N; Hedrich, Hans-J; Gunzer, Florian
Abstract:
Escherichia coli Nissle 1917 (EcN) is a well-characterized probiotic bacterium. Although genomic comparisons of EcN with the uropathogenic E. coli strain CFT073 revealed high degrees of similarity, EcN is generally considered a non-pathogenic organism. However, as recent evidence suggests that EcN is capable of inducing inflammatory responses in host intestinal epithelial cells, we aimed to investigate potential pathogenic properties of EcN in an in vivo model using various germ-free (GF) mouse strains. With the exception of C3H/HeJZtm mice, which carry a defective toll-like receptor (TLR)4-allele, no lesions were obvious in mice of different strains orally inoculated with EcN for 1 week, although organ cultures (blood, lung, mesenteric lymph node, pancreas, spleen, liver and kidney) tested positive to various degrees. C3H/HeJZtm mice inoculated with EcN became clinically ill and the majority died or had to be euthanized. Organs of all gnotobiotic C3H/HeJZtm mice were positive for EcN by culture; major histological findings were moderate to severe pyogranulomatous serositis, typhlitis and pancreatitis. Histological findings were corroborated by highly elevated tumour necrosis factor (TNF) serum levels. Lesions were not detected in specified pathogen free maintained C3H/HeJZtm mice, GF C3H/HeJ mice lacking the interleukin-10 gene, or GF C3H/HeJZtm mice that were inoculated with E. coli K12 strain MG1655 as a control. In addition, mild histological lesions were detected in Ztm:NMRI mice 3 months after oral inoculation with EcN. This study shows that EcN is capable of displaying a virulent phenotype in GF C3H/HeJZtm mice. Whether this phenotype is linked to the bacterium's probiotic nature should be the focus of further studies.
Affiliation:
Institute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Hannover, Germany. bleich.andre@mh-hannover.de
Citation:
Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background. 2008, 89 (1):45-54notInt J Exp Pathol
Journal:
International journal of experimental pathology
Issue Date:
Feb-2008
URI:
http://hdl.handle.net/10033/28152
DOI:
10.1111/j.1365-2613.2007.00560.x
PubMed ID:
18005134
Type:
Article
Language:
en
ISSN:
1365-2613
Appears in Collections:
Publications of RG Mucosal Immunity (MI)

Full metadata record

DC FieldValue Language
dc.contributor.authorBleich, Andre-
dc.contributor.authorSundberg, John P-
dc.contributor.authorSmoczek, Anna-
dc.contributor.authorvon Wasielewski, Reinhard-
dc.contributor.authorde Buhr, Maike F-
dc.contributor.authorJanus, Lydia M-
dc.contributor.authorJulga, Gwen-
dc.contributor.authorUkena, Sya N-
dc.contributor.authorHedrich, Hans-J-
dc.contributor.authorGunzer, Florian-
dc.date.accessioned2008-05-26T13:15:34Z-
dc.date.available2008-05-26T13:15:34Z-
dc.date.issued2008-02-
dc.identifier.citationSensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background. 2008, 89 (1):45-54notInt J Exp Patholen
dc.identifier.issn1365-2613-
dc.identifier.pmid18005134-
dc.identifier.doi10.1111/j.1365-2613.2007.00560.x-
dc.identifier.urihttp://hdl.handle.net/10033/28152-
dc.description.abstractEscherichia coli Nissle 1917 (EcN) is a well-characterized probiotic bacterium. Although genomic comparisons of EcN with the uropathogenic E. coli strain CFT073 revealed high degrees of similarity, EcN is generally considered a non-pathogenic organism. However, as recent evidence suggests that EcN is capable of inducing inflammatory responses in host intestinal epithelial cells, we aimed to investigate potential pathogenic properties of EcN in an in vivo model using various germ-free (GF) mouse strains. With the exception of C3H/HeJZtm mice, which carry a defective toll-like receptor (TLR)4-allele, no lesions were obvious in mice of different strains orally inoculated with EcN for 1 week, although organ cultures (blood, lung, mesenteric lymph node, pancreas, spleen, liver and kidney) tested positive to various degrees. C3H/HeJZtm mice inoculated with EcN became clinically ill and the majority died or had to be euthanized. Organs of all gnotobiotic C3H/HeJZtm mice were positive for EcN by culture; major histological findings were moderate to severe pyogranulomatous serositis, typhlitis and pancreatitis. Histological findings were corroborated by highly elevated tumour necrosis factor (TNF) serum levels. Lesions were not detected in specified pathogen free maintained C3H/HeJZtm mice, GF C3H/HeJ mice lacking the interleukin-10 gene, or GF C3H/HeJZtm mice that were inoculated with E. coli K12 strain MG1655 as a control. In addition, mild histological lesions were detected in Ztm:NMRI mice 3 months after oral inoculation with EcN. This study shows that EcN is capable of displaying a virulent phenotype in GF C3H/HeJZtm mice. Whether this phenotype is linked to the bacterium's probiotic nature should be the focus of further studies.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshDisease Models, Animalen
dc.subject.meshEscherichia colien
dc.subject.meshFemaleen
dc.subject.meshGerm-Free Lifeen
dc.subject.meshIntestinesen
dc.subject.meshMaleen
dc.subject.meshMiceen
dc.subject.meshProbioticsen
dc.subject.meshSensitivity and Specificityen
dc.titleSensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background.en
dc.typeArticleen
dc.contributor.departmentInstitute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Hannover, Germany. bleich.andre@mh-hannover.deen
dc.identifier.journalInternational journal of experimental pathologyen

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