2.50
Hdl Handle:
http://hdl.handle.net/10033/293025
Title:
Immunoglobulins drive terminal maturation of splenic dendritic cells.
Authors:
Zietara, Natalia; Łyszkiewicz, Marcin; Puchałka, Jacek; Pei, Gang; Gutierrez, Maximiliano Gabriel; Lienenklaus, Stefan; Hobeika, Elias; Reth, Michael; Martins dos Santos, Vitor A P; Krueger, Andreas; Weiss, Siegfried
Abstract:
Nature and physiological status of antigen-presenting cells, such as dendritic cells DCs, are decisive for the immune reactions elicited. Multiple factors and cell interactions have been described that affect maturation of DCs. Here, we show that DCs arising in the absence of immunoglobulins (Ig) in vivo are impaired in cross-presentation of soluble antigen. This deficiency was due to aberrant cellular targeting of antigen to lysosomes and its rapid degradation. Function of DCs could be restored by transfer of Ig irrespective of antigen specificity and isotype. Modulation of cross-presentation by Ig was inhibited by coapplication of mannan and, thus, likely to be mediated by C-type lectin receptors. This unexpected dependency of splenic DCs on Ig to cross-present antigen provides insights into the interplay between cellular and humoral immunity and the immunomodulatory capacity of Ig.
Affiliation:
Department of Molecular Immunology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany. zietara.natalia@mh-hannover.de
Citation:
Immunoglobulins drive terminal maturation of splenic dendritic cells. 2013, 110 (6):2282-7 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
5-Feb-2013
URI:
http://hdl.handle.net/10033/293025
DOI:
10.1073/pnas.1210654110
PubMed ID:
23345431
Type:
Article
Language:
en
ISSN:
1091-6490
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorZietara, Nataliaen_GB
dc.contributor.authorŁyszkiewicz, Marcinen_GB
dc.contributor.authorPuchałka, Jaceken_GB
dc.contributor.authorPei, Gangen_GB
dc.contributor.authorGutierrez, Maximiliano Gabrielen_GB
dc.contributor.authorLienenklaus, Stefanen_GB
dc.contributor.authorHobeika, Eliasen_GB
dc.contributor.authorReth, Michaelen_GB
dc.contributor.authorMartins dos Santos, Vitor A Pen_GB
dc.contributor.authorKrueger, Andreasen_GB
dc.contributor.authorWeiss, Siegfrieden_GB
dc.date.accessioned2013-05-30T09:25:25Z-
dc.date.available2013-05-30T09:25:25Z-
dc.date.issued2013-02-05-
dc.identifier.citationImmunoglobulins drive terminal maturation of splenic dendritic cells. 2013, 110 (6):2282-7 Proc. Natl. Acad. Sci. U.S.A.en_GB
dc.identifier.issn1091-6490-
dc.identifier.pmid23345431-
dc.identifier.doi10.1073/pnas.1210654110-
dc.identifier.urihttp://hdl.handle.net/10033/293025-
dc.description.abstractNature and physiological status of antigen-presenting cells, such as dendritic cells DCs, are decisive for the immune reactions elicited. Multiple factors and cell interactions have been described that affect maturation of DCs. Here, we show that DCs arising in the absence of immunoglobulins (Ig) in vivo are impaired in cross-presentation of soluble antigen. This deficiency was due to aberrant cellular targeting of antigen to lysosomes and its rapid degradation. Function of DCs could be restored by transfer of Ig irrespective of antigen specificity and isotype. Modulation of cross-presentation by Ig was inhibited by coapplication of mannan and, thus, likely to be mediated by C-type lectin receptors. This unexpected dependency of splenic DCs on Ig to cross-present antigen provides insights into the interplay between cellular and humoral immunity and the immunomodulatory capacity of Ig.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Proceedings of the National Academy of Sciences of the United States of Americaen_GB
dc.subject.meshAnimalsen_GB
dc.subject.meshB-Lymphocytesen_GB
dc.subject.meshCell Differentiationen_GB
dc.subject.meshCross-Primingen_GB
dc.subject.meshDendritic Cellsen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshImmunoglobulinsen_GB
dc.subject.meshLectins, C-Typeen_GB
dc.subject.meshLymphopeniaen_GB
dc.subject.meshMiceen_GB
dc.subject.meshMice, Inbred C57BLen_GB
dc.subject.meshMice, Knockouten_GB
dc.subject.meshSpleenen_GB
dc.subject.meshT-Lymphocytesen_GB
dc.titleImmunoglobulins drive terminal maturation of splenic dendritic cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Immunology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany. zietara.natalia@mh-hannover.deen_GB
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen_GB

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