Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice.

2.50
Hdl Handle:
http://hdl.handle.net/10033/293072
Title:
Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice.
Authors:
Bergmann, Silke; Beard, Philippa M; Pasche, Bastian; Lienenklaus, Stefan; Weiss, Siegfried; Gahan, Cormac G M; Schughart, Klaus ( 0000-0002-6824-7523 ) ; Lengeling, Andreas
Abstract:
The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of 'murinisation' to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised Listeria strains to examine the spatiotemporal dissemination of Listeria in four diverse mouse genetic backgrounds after oral inoculation.
Affiliation:
Infection and Immunity Division, The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush Veterinary Campus, Edinburgh EH25 9RG, UK. andreas.lengeling@roslin.ed.ac.uk.
Citation:
Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. 2013, 13:90 BMC Microbiol.
Journal:
BMC microbiology
Issue Date:
2013
URI:
http://hdl.handle.net/10033/293072
DOI:
10.1186/1471-2180-13-90
PubMed ID:
23617550
Type:
Article
Language:
en
ISSN:
1471-2180
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK); publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorBergmann, Silkeen_GB
dc.contributor.authorBeard, Philippa Men_GB
dc.contributor.authorPasche, Bastianen_GB
dc.contributor.authorLienenklaus, Stefanen_GB
dc.contributor.authorWeiss, Siegfrieden_GB
dc.contributor.authorGahan, Cormac G Men_GB
dc.contributor.authorSchughart, Klausen_GB
dc.contributor.authorLengeling, Andreasen_GB
dc.date.accessioned2013-05-30T14:12:07Zen
dc.date.available2013-05-30T14:12:07Zen
dc.date.issued2013en
dc.identifier.citationInfluence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. 2013, 13:90 BMC Microbiol.en_GB
dc.identifier.issn1471-2180en
dc.identifier.pmid23617550en
dc.identifier.doi10.1186/1471-2180-13-90en
dc.identifier.urihttp://hdl.handle.net/10033/293072en
dc.description.abstractThe bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of 'murinisation' to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised Listeria strains to examine the spatiotemporal dissemination of Listeria in four diverse mouse genetic backgrounds after oral inoculation.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to BMC microbiologyen_GB
dc.titleInfluence of internalin a murinisation on host resistance to orally acquired listeriosis in mice.en
dc.typeArticleen
dc.contributor.departmentInfection and Immunity Division, The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush Veterinary Campus, Edinburgh EH25 9RG, UK. andreas.lengeling@roslin.ed.ac.uk.en_GB
dc.identifier.journalBMC microbiologyen_GB

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