2.50
Hdl Handle:
http://hdl.handle.net/10033/293126
Title:
PfaH2: A novel hydrophobin from the ascomycete Paecilomyces farinosus.
Authors:
Zelena, Katerina; Takenberg, Meike; Lunkenbein, Stefan; Woche, Susanne K; Nimtz, Manfred; Berger, Ralf G
Abstract:
The pfah2 gene coding for a novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified during sequencing of random clones from a cDNA library. The corresponding protein sequence of PfaH2 deduced from the cDNA comprised 134 amino acids (aa). A 16 aa signal sequence preceded the N-terminus of the mature protein. PfaH2 belonged to the class Ia hydrophobins. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. The surface activity of the recently described PfaH1 and of PfaH2 was compared by the determination of contact angles (CAs) on glass slides and Teflon tape, and the CA of distilled water droplets was measured on glass slides coated with hydrophobin PfaH1 or PfaH2. Surprisingly, both hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to a similar quantitative extent, although little aa sequence homology was found.
Affiliation:
Naturwissenschaftliche Fakultät der Leibniz Universität Hannover, Institut für Lebensmittelchemie, Hannover, Germany.
Citation:
PfaH2: A novel hydrophobin from the ascomycete Paecilomyces farinosus. 2013, 60 (2):147-54 Biotechnol. Appl. Biochem.
Journal:
Biotechnology and applied biochemistry
Issue Date:
Mar-2013
URI:
http://hdl.handle.net/10033/293126
DOI:
10.1002/bab.1077
PubMed ID:
23600571
Type:
Article
Language:
en
ISSN:
1470-8744
Appears in Collections:
Publications from Division of Molekulare Struktur Biologie (MOSB)

Full metadata record

DC FieldValue Language
dc.contributor.authorZelena, Katerinaen_GB
dc.contributor.authorTakenberg, Meikeen_GB
dc.contributor.authorLunkenbein, Stefanen_GB
dc.contributor.authorWoche, Susanne Ken_GB
dc.contributor.authorNimtz, Manfreden_GB
dc.contributor.authorBerger, Ralf Gen_GB
dc.date.accessioned2013-05-31T09:30:11Z-
dc.date.available2013-05-31T09:30:11Z-
dc.date.issued2013-03-
dc.identifier.citationPfaH2: A novel hydrophobin from the ascomycete Paecilomyces farinosus. 2013, 60 (2):147-54 Biotechnol. Appl. Biochem.en_GB
dc.identifier.issn1470-8744-
dc.identifier.pmid23600571-
dc.identifier.doi10.1002/bab.1077-
dc.identifier.urihttp://hdl.handle.net/10033/293126-
dc.description.abstractThe pfah2 gene coding for a novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified during sequencing of random clones from a cDNA library. The corresponding protein sequence of PfaH2 deduced from the cDNA comprised 134 amino acids (aa). A 16 aa signal sequence preceded the N-terminus of the mature protein. PfaH2 belonged to the class Ia hydrophobins. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. The surface activity of the recently described PfaH1 and of PfaH2 was compared by the determination of contact angles (CAs) on glass slides and Teflon tape, and the CA of distilled water droplets was measured on glass slides coated with hydrophobin PfaH1 or PfaH2. Surprisingly, both hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to a similar quantitative extent, although little aa sequence homology was found.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Biotechnology and applied biochemistryen_GB
dc.titlePfaH2: A novel hydrophobin from the ascomycete Paecilomyces farinosus.en
dc.typeArticleen
dc.contributor.departmentNaturwissenschaftliche Fakultät der Leibniz Universität Hannover, Institut für Lebensmittelchemie, Hannover, Germany.en_GB
dc.identifier.journalBiotechnology and applied biochemistryen_GB

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