2.50
Hdl Handle:
http://hdl.handle.net/10033/294326
Title:
New lineage of filamentous, spore-forming, gram-positive bacteria from soil.
Authors:
Cavaletti, Linda; Monciardini, Paolo; Bamonte, Ruggiero; Schumann, Peter; Rohde, Manfred; Sosio, Margherita; Donadio, Stefano
Abstract:
A novel bacterial strain that was isolated from an Italian soil and was designated SOSP1-21T forms branched mycelia in solid and liquid media and has a filamentous morphology similar to that of some genera belonging to the Actinobacteria. Electron microscopy showed that this organism has a grape-like appearance, resulting from interlacing of spores originating from sporophoric hyphae. Ten strains that are morphologically related to SOSP1-21T were recovered from soil. Phylogenetic analyses of 16S rRNA gene segments confirmed the relatedness of these strains to SOSP1-21T and indicated that the newly isolated strains form separate clades in a deeply branching lineage. The closest matches for the 16S rRNA sequences of all the strains (around 79% identity) were matches with representatives of the Chloroflexi, although the affiliation with this division was not supported by high bootstrap values. The strains are mesophilic aerobic heterotrophs and are also capable of growing under microaerophilic conditions. They all stain gram positive. Strain SOSP1-21T contains ornithine, alanine, glutamic acid, serine, and glycine as the peptidoglycan amino acids. In addition, an unusual level of C16:1 2OH (30%) was found in the cellular fatty acids. The G+C content of SOSP1-21T genomic DNA is 53.9%, and MK-9(H2) was the only menaquinone detected. All these data suggest that SOSP1-21T and the related strains may constitute a new division of filamentous, spore-forming, gram-positive bacteria. We propose the name Ktedobacter racemifer gen. nov., sp. nov. for strain SOSP1-21T.
Affiliation:
Vicuron Pharmaceuticals, 21040 Gerenzano, Italy.
Citation:
New lineage of filamentous, spore-forming, gram-positive bacteria from soil. 2006, 72 (6):4360-9 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
Jun-2006
URI:
http://hdl.handle.net/10033/294326
DOI:
10.1128/AEM.00132-06
PubMed ID:
16751552
Type:
Article
Language:
en
ISSN:
0099-2240
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValue Language
dc.contributor.authorCavaletti, Lindaen_GB
dc.contributor.authorMonciardini, Paoloen_GB
dc.contributor.authorBamonte, Ruggieroen_GB
dc.contributor.authorSchumann, Peteren_GB
dc.contributor.authorRohde, Manfreden_GB
dc.contributor.authorSosio, Margheritaen_GB
dc.contributor.authorDonadio, Stefanoen_GB
dc.date.accessioned2013-06-21T10:22:40Zen
dc.date.available2013-06-21T10:22:40Zen
dc.date.issued2006-06en
dc.identifier.citationNew lineage of filamentous, spore-forming, gram-positive bacteria from soil. 2006, 72 (6):4360-9 Appl. Environ. Microbiol.en_GB
dc.identifier.issn0099-2240en
dc.identifier.pmid16751552en
dc.identifier.doi10.1128/AEM.00132-06en
dc.identifier.urihttp://hdl.handle.net/10033/294326en
dc.description.abstractA novel bacterial strain that was isolated from an Italian soil and was designated SOSP1-21T forms branched mycelia in solid and liquid media and has a filamentous morphology similar to that of some genera belonging to the Actinobacteria. Electron microscopy showed that this organism has a grape-like appearance, resulting from interlacing of spores originating from sporophoric hyphae. Ten strains that are morphologically related to SOSP1-21T were recovered from soil. Phylogenetic analyses of 16S rRNA gene segments confirmed the relatedness of these strains to SOSP1-21T and indicated that the newly isolated strains form separate clades in a deeply branching lineage. The closest matches for the 16S rRNA sequences of all the strains (around 79% identity) were matches with representatives of the Chloroflexi, although the affiliation with this division was not supported by high bootstrap values. The strains are mesophilic aerobic heterotrophs and are also capable of growing under microaerophilic conditions. They all stain gram positive. Strain SOSP1-21T contains ornithine, alanine, glutamic acid, serine, and glycine as the peptidoglycan amino acids. In addition, an unusual level of C16:1 2OH (30%) was found in the cellular fatty acids. The G+C content of SOSP1-21T genomic DNA is 53.9%, and MK-9(H2) was the only menaquinone detected. All these data suggest that SOSP1-21T and the related strains may constitute a new division of filamentous, spore-forming, gram-positive bacteria. We propose the name Ktedobacter racemifer gen. nov., sp. nov. for strain SOSP1-21T.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Applied and environmental microbiologyen_GB
dc.subject.meshFatty Acidsen_GB
dc.subject.meshGram-Positive Bacteriaen_GB
dc.subject.meshItalyen_GB
dc.subject.meshMicroscopy, Electronen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshPhylogenyen_GB
dc.subject.meshSoil Microbiologyen_GB
dc.subject.meshSpores, Bacterialen_GB
dc.titleNew lineage of filamentous, spore-forming, gram-positive bacteria from soil.en
dc.typeArticleen
dc.contributor.departmentVicuron Pharmaceuticals, 21040 Gerenzano, Italy.en_GB
dc.identifier.journalApplied and environmental microbiologyen_GB

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