2.50
Hdl Handle:
http://hdl.handle.net/10033/294443
Title:
Freeze-drying as a preserving preparation technique for in vitro testing of human skin.
Authors:
Franzen, Lutz; Vidlářová, Lucie; Kostka, Karl-Heinz; Schaefer, Ulrich F; Windbergs, Maike
Abstract:
In vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.
Citation:
Freeze-drying as a preserving preparation technique for in vitro testing of human skin. 2013, 22 (1):54-6 Exp. Dermatol.
Journal:
Experimental dermatology
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10033/294443
DOI:
10.1111/exd.12058
PubMed ID:
23278895
Type:
Article
Language:
en
ISSN:
1600-0625
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorFranzen, Lutzen_GB
dc.contributor.authorVidlářová, Lucieen_GB
dc.contributor.authorKostka, Karl-Heinzen_GB
dc.contributor.authorSchaefer, Ulrich Fen_GB
dc.contributor.authorWindbergs, Maikeen_GB
dc.date.accessioned2013-06-24T14:25:15Z-
dc.date.available2013-06-24T14:25:15Z-
dc.date.issued2013-01-
dc.identifier.citationFreeze-drying as a preserving preparation technique for in vitro testing of human skin. 2013, 22 (1):54-6 Exp. Dermatol.en_GB
dc.identifier.issn1600-0625-
dc.identifier.pmid23278895-
dc.identifier.doi10.1111/exd.12058-
dc.identifier.urihttp://hdl.handle.net/10033/294443-
dc.description.abstractIn vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze-drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze-drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze-drying. Furthermore, we show that freeze-drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze-drying as a preserving preparation technique for in vitro testing of human skin.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Experimental dermatologyen_GB
dc.titleFreeze-drying as a preserving preparation technique for in vitro testing of human skin.en
dc.typeArticleen
dc.identifier.journalExperimental dermatologyen_GB

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