In vitro activity of colistin as single agent and in combination with antifungals against filamentous fungi occurring in patients with cystic fibrosis.

2.50
Hdl Handle:
http://hdl.handle.net/10033/294772
Title:
In vitro activity of colistin as single agent and in combination with antifungals against filamentous fungi occurring in patients with cystic fibrosis.
Authors:
Schemuth, H; Dittmer, S; Lackner, M; Sedlacek, L; Hamprecht, A; Steinmann, E; Buer, J; Rath, P-M; Steinmann, J
Abstract:
Because published reports indicate that the antibiotic colistin (COL) has antifungal properties, this study investigated the antifungal in vitro activity of COL as single agent and in combination with the antifungal compounds voriconazole (VRC), caspofungin (CAS) and amphotericin B (AMB) against Scedosporium/Pseudallescheria spp., Exophiala dermatitidis and Geosmithia argillacea. In total, susceptibility was determined for 77 Scedosporium/Pseudallescheria spp., 82 E. dermatitidis and 17 G. argillacea isolates. The minimal inhibitory concentrations (MICs) of COL and the antifungals as single compound and in combination were determined with MIC test strips. Drug interactions were detected by crossing the MIC test strips at a 90º angle. The fractional inhibitory concentration index was used to categorise the drugs' interaction. The MIC50 value of COL was 12 μg ml(-1) for S. prolificans, 16 μg ml(-1) for P. apiosperma, 16 μg ml(-1) for P. boydii, 12 μg ml(-1) for E. dermatiditis and 6 μg ml(-1) for G. argillacea. VRC was the most active drug in combination without any antagonism with the exception of few P. boydii isolates. COL as single agent and in most combinations with antifungals exhibits in vitro antifungal activity against filamentous ascomycetes occurring in cystic fibrosis patients and may offer a novel therapeutic option, especially for multidrug-resistant S. prolificans.
Affiliation:
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, Essen, Germany.
Citation:
In vitro activity of colistin as single agent and in combination with antifungals against filamentous fungi occurring in patients with cystic fibrosis. 2013, 56 (3):297-303 Mycoses
Journal:
Mycoses
Issue Date:
May-2013
URI:
http://hdl.handle.net/10033/294772
DOI:
10.1111/myc.12022
PubMed ID:
23170818
Type:
Article
Language:
en
ISSN:
1439-0507
Appears in Collections:
publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchemuth, Hen_GB
dc.contributor.authorDittmer, Sen_GB
dc.contributor.authorLackner, Men_GB
dc.contributor.authorSedlacek, Len_GB
dc.contributor.authorHamprecht, Aen_GB
dc.contributor.authorSteinmann, Een_GB
dc.contributor.authorBuer, Jen_GB
dc.contributor.authorRath, P-Men_GB
dc.contributor.authorSteinmann, Jen_GB
dc.date.accessioned2013-06-27T12:29:21Zen
dc.date.available2013-06-27T12:29:21Zen
dc.date.issued2013-05en
dc.identifier.citationIn vitro activity of colistin as single agent and in combination with antifungals against filamentous fungi occurring in patients with cystic fibrosis. 2013, 56 (3):297-303 Mycosesen_GB
dc.identifier.issn1439-0507en
dc.identifier.pmid23170818en
dc.identifier.doi10.1111/myc.12022en
dc.identifier.urihttp://hdl.handle.net/10033/294772en
dc.description.abstractBecause published reports indicate that the antibiotic colistin (COL) has antifungal properties, this study investigated the antifungal in vitro activity of COL as single agent and in combination with the antifungal compounds voriconazole (VRC), caspofungin (CAS) and amphotericin B (AMB) against Scedosporium/Pseudallescheria spp., Exophiala dermatitidis and Geosmithia argillacea. In total, susceptibility was determined for 77 Scedosporium/Pseudallescheria spp., 82 E. dermatitidis and 17 G. argillacea isolates. The minimal inhibitory concentrations (MICs) of COL and the antifungals as single compound and in combination were determined with MIC test strips. Drug interactions were detected by crossing the MIC test strips at a 90º angle. The fractional inhibitory concentration index was used to categorise the drugs' interaction. The MIC50 value of COL was 12 μg ml(-1) for S. prolificans, 16 μg ml(-1) for P. apiosperma, 16 μg ml(-1) for P. boydii, 12 μg ml(-1) for E. dermatiditis and 6 μg ml(-1) for G. argillacea. VRC was the most active drug in combination without any antagonism with the exception of few P. boydii isolates. COL as single agent and in most combinations with antifungals exhibits in vitro antifungal activity against filamentous ascomycetes occurring in cystic fibrosis patients and may offer a novel therapeutic option, especially for multidrug-resistant S. prolificans.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Mycosesen_GB
dc.titleIn vitro activity of colistin as single agent and in combination with antifungals against filamentous fungi occurring in patients with cystic fibrosis.en
dc.typeArticleen
dc.contributor.departmentInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, Essen, Germany.en_GB
dc.identifier.journalMycosesen_GB

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