Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data.

2.50
Hdl Handle:
http://hdl.handle.net/10033/297445
Title:
Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data.
Authors:
Labouta, Hagar I; Thude, Sibylle; Schneider, Marc ( 0000-0002-9260-7357 )
Abstract:
Owing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λ(ex)=800 nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.
Affiliation:
Helmholtz Center for Infection Research, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany.
Citation:
Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data. 2013, 18 (6):061218 J Biomed Opt
Journal:
Journal of biomedical optics
Issue Date:
Jun-2013
URI:
http://hdl.handle.net/10033/297445
DOI:
10.1117/1.JBO.18.6.061218
PubMed ID:
23203297
Type:
Article
Language:
en
ISSN:
1560-2281
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorLabouta, Hagar Ien_GB
dc.contributor.authorThude, Sibylleen_GB
dc.contributor.authorSchneider, Marcen_GB
dc.date.accessioned2013-08-06T09:20:32Zen
dc.date.available2013-08-06T09:20:32Zen
dc.date.issued2013-06en
dc.identifier.citationSetup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data. 2013, 18 (6):061218 J Biomed Opten_GB
dc.identifier.issn1560-2281en
dc.identifier.pmid23203297en
dc.identifier.doi10.1117/1.JBO.18.6.061218en
dc.identifier.urihttp://hdl.handle.net/10033/297445en
dc.description.abstractOwing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λ(ex)=800 nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of biomedical opticsen_GB
dc.subject.meshBiomedical Researchen_GB
dc.subject.meshCell Culture Techniquesen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshFibroblastsen_GB
dc.subject.meshGolden_GB
dc.subject.meshHumansen_GB
dc.subject.meshKeratinocytesen_GB
dc.subject.meshMetal Nanoparticlesen_GB
dc.subject.meshMicroscopy, Fluorescence, Multiphotonen_GB
dc.subject.meshModels, Biologicalen_GB
dc.subject.meshPermeabilityen_GB
dc.subject.meshReproducibility of Resultsen_GB
dc.subject.meshSkinen_GB
dc.titleSetup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Center for Infection Research, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany.en_GB
dc.identifier.journalJournal of biomedical opticsen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.