Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling.
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Authors
Wodke, Judith A HPuchałka, Jacek
Lluch-Senar, Maria
Marcos, Josep
Yus, Eva
Godinho, Miguel
Gutiérrez-Gallego, Ricardo
dos Santos, Vitor A P Martins
Serrano, Luis
Klipp, Edda
Maier, Tobias
Issue Date
2013
Metadata
Show full item recordAbstract
Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism for bacterial systems biology for which substantial experimental information is available. With the goal of understanding the complex interactions underlying its metabolism, we analyzed and characterized the metabolic network of M. pneumoniae in great detail, integrating data from different omics analyses under a range of conditions into a constraint-based model backbone. Iterating model predictions, hypothesis generation, experimental testing, and model refinement, we accurately curated the network and quantitatively explored the energy metabolism. In contrast to other bacteria, M. pneumoniae uses most of its energy for maintenance tasks instead of growth. We show that in highly linear networks the prediction of flux distributions for different growth times allows analysis of time-dependent changes, albeit using a static model. By performing an in silico knock-out study as well as analyzing flux distributions in single and double mutant phenotypes, we demonstrated that the model accurately represents the metabolism of M. pneumoniae. The experimentally validated model provides a solid basis for understanding its metabolic regulatory mechanisms.Citation
Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling. 2013, 9:653 Mol. Syst. Biol.Affiliation
EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), Barcelona, Spain.Journal
Molecular systems biologyPubMed ID
23549481Type
ArticleLanguage
enISSN
1744-4292ae974a485f413a2113503eed53cd6c53
10.1038/msb.2013.6
Scopus Count
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