2.50
Hdl Handle:
http://hdl.handle.net/10033/303152
Title:
Proteome analysis of distinct developmental stages of human natural killer (NK) cells.
Authors:
Scheiter, Maxi; Lau, Ulrike; van Ham, Marco; Bulitta, Björn; Gröbe, Lothar; Garritsen, Henk; Klawonn, Frank; König, Sebastian; Jänsch, Lothar ( 0000-0002-5655-1181 )
Abstract:
The recent Natural Killer (NK) cell maturation model postulates that CD34(+) hematopoietic stem cells (HSC) first develop into CD56(bright) NK cells, then into CD56(dim)CD57(-) and finally into terminally maturated CD56(dim)CD57(+). The molecular mechanisms of human NK cell differentiation and maturation however are incompletely characterized. Here we present a proteome analysis of distinct developmental stages of human primary NK cells, isolated from healthy human blood donors. Peptide sequencing was used to comparatively analyze CD56(bright) NK cells versus CD56(dim) NK cells and CD56(dim)CD57(-) NK cells versus CD56(dim)CD57(+) NK cells and revealed distinct protein signatures for all of these subsets. Quantitative data for about 3400 proteins were obtained and support the current differentiation model. Furthermore, 11 donor-independently, but developmental stage specifically regulated proteins so far undescribed in NK cells were revealed, which may contribute to NK cell development and may elucidate a molecular source for NK cell effector functions. Among those proteins, S100A4 (Calvasculin) and S100A6 (Calcyclin) were selected to study their dynamic subcellular localization. Upon activation of human primary NK cells, both proteins are recruited into the immune synapse (NKIS), where they colocalize with myosin IIa.
Affiliation:
Research Group Cellular Proteomics, Helmholtz Centre for Infection Research, HZI, Inhoffenstraβe 7, D-38124 Braunschweig, Germany.
Citation:
Proteome analysis of distinct developmental stages of human natural killer (NK) cells. 2013, 12 (5):1099-114 Mol. Cell Proteomics
Journal:
Molecular & cellular proteomics : MCP
Issue Date:
May-2013
URI:
http://hdl.handle.net/10033/303152
DOI:
10.1074/mcp.M112.024596
PubMed ID:
23315794
Type:
Article
Language:
en
ISSN:
1535-9484
Appears in Collections:
publications of the research group cellular proteom research (CPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorScheiter, Maxien
dc.contributor.authorLau, Ulrikeen
dc.contributor.authorvan Ham, Marcoen
dc.contributor.authorBulitta, Björnen
dc.contributor.authorGröbe, Lotharen
dc.contributor.authorGarritsen, Henken
dc.contributor.authorKlawonn, Franken
dc.contributor.authorKönig, Sebastianen
dc.contributor.authorJänsch, Lotharen
dc.date.accessioned2013-10-10T11:15:20Zen
dc.date.available2013-10-10T11:15:20Zen
dc.date.issued2013-05en
dc.identifier.citationProteome analysis of distinct developmental stages of human natural killer (NK) cells. 2013, 12 (5):1099-114 Mol. Cell Proteomicsen
dc.identifier.issn1535-9484en
dc.identifier.pmid23315794en
dc.identifier.doi10.1074/mcp.M112.024596en
dc.identifier.urihttp://hdl.handle.net/10033/303152en
dc.description.abstractThe recent Natural Killer (NK) cell maturation model postulates that CD34(+) hematopoietic stem cells (HSC) first develop into CD56(bright) NK cells, then into CD56(dim)CD57(-) and finally into terminally maturated CD56(dim)CD57(+). The molecular mechanisms of human NK cell differentiation and maturation however are incompletely characterized. Here we present a proteome analysis of distinct developmental stages of human primary NK cells, isolated from healthy human blood donors. Peptide sequencing was used to comparatively analyze CD56(bright) NK cells versus CD56(dim) NK cells and CD56(dim)CD57(-) NK cells versus CD56(dim)CD57(+) NK cells and revealed distinct protein signatures for all of these subsets. Quantitative data for about 3400 proteins were obtained and support the current differentiation model. Furthermore, 11 donor-independently, but developmental stage specifically regulated proteins so far undescribed in NK cells were revealed, which may contribute to NK cell development and may elucidate a molecular source for NK cell effector functions. Among those proteins, S100A4 (Calvasculin) and S100A6 (Calcyclin) were selected to study their dynamic subcellular localization. Upon activation of human primary NK cells, both proteins are recruited into the immune synapse (NKIS), where they colocalize with myosin IIa.en
dc.language.isoenen
dc.rightsArchived with thanks to Molecular & cellular proteomics : MCPen
dc.titleProteome analysis of distinct developmental stages of human natural killer (NK) cells.en
dc.typeArticleen
dc.contributor.departmentResearch Group Cellular Proteomics, Helmholtz Centre for Infection Research, HZI, Inhoffenstraβe 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalMolecular & cellular proteomics : MCPen

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