2.50
Hdl Handle:
http://hdl.handle.net/10033/304813
Title:
Complete sequence of the suicide vector pJP5603.
Authors:
Riedel, Thomas; Rohlfs, Meike; Buchholz, Ina; Wagner-Döbler, Irene; Reck, Michael
Abstract:
We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.
Affiliation:
Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.de
Citation:
Complete sequence of the suicide vector pJP5603. 2013, 69 (1):104-7 Plasmid
Journal:
Plasmid
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10033/304813
DOI:
10.1016/j.plasmid.2012.07.005
PubMed ID:
22902299
Type:
Article
Language:
en
ISSN:
1095-9890
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorRiedel, Thomasen
dc.contributor.authorRohlfs, Meikeen
dc.contributor.authorBuchholz, Inaen
dc.contributor.authorWagner-Döbler, Ireneen
dc.contributor.authorReck, Michaelen
dc.date.accessioned2013-10-31T12:38:37Z-
dc.date.available2013-10-31T12:38:37Z-
dc.date.issued2013-01-
dc.identifier.citationComplete sequence of the suicide vector pJP5603. 2013, 69 (1):104-7 Plasmiden
dc.identifier.issn1095-9890-
dc.identifier.pmid22902299-
dc.identifier.doi10.1016/j.plasmid.2012.07.005-
dc.identifier.urihttp://hdl.handle.net/10033/304813-
dc.description.abstractWe have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.en
dc.language.isoenen
dc.rightsArchived with thanks to Plasmiden
dc.subject.meshBacterial Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshChromosomesen
dc.subject.meshCloning, Molecularen
dc.subject.meshDNA Replicationen
dc.subject.meshEscherichia colien
dc.subject.meshGenetic Vectorsen
dc.subject.meshHomologous Recombinationen
dc.subject.meshKanamycinen
dc.subject.meshLac Operonen
dc.subject.meshMutagenesis, Site-Directeden
dc.subject.meshPlasmidsen
dc.titleComplete sequence of the suicide vector pJP5603.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.deen
dc.identifier.journalPlasmiden
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