2.50
Hdl Handle:
http://hdl.handle.net/10033/338595
Title:
New insights into the antimicrobial effect of mast cells against Enterococcus faecalis.
Authors:
Scheb-Wetzel, Matthias; Rohde, Manfred; Bravo, Alicia; Goldmann, Oliver
Abstract:
Enterococcus faecalis has emerged as an important cause of life-threatening multidrug-resistant bacterial infections in the hospital setting. The pathogenesis of enterococcal infections has remained a relatively neglected field despite their obvious clinical relevance. The objective of this study was to characterize the interactions between mast cells (MCs), an innate immune cell population abundant in the intestinal lamina propria, and E. faecalis. This study was conducted with primary bone marrow-derived murine MCs. The results demonstrated that MCs exerted an antimicrobial effect against E. faecalis that was mediated both by degranulation, with the concomitant discharge of the antimicrobial effectors contained in the granules, and by the release of extracellular traps, in which E. faecalis was snared and killed. In particular, the cathelicidin LL-37 released by the MCs had potent antimicrobial effect against E. faecalis. We also investigated the specific receptors involved in the recognition of E. faecalis by MCs. We found that Toll-like receptors (TLRs) are critically involved in the MC recognition of E. faecalis, since MCs deficient in the expression of MyD88, an adaptor molecule required for signaling by most TLRs, were significantly impaired in their capacity to degranulate, to reduce E. faecalis growth as well as to release tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) after encountering this pathogen. Furthermore, TLR2 was identified as the most prominent TLR involved in the recognition of E. faecalis by MCs. The results of this study indicate that MCs may be important contributors to the host innate immune defenses against E. faecalis.
Affiliation:
Helmholtz Centre for infection reseach,Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
New insights into the antimicrobial effect of mast cells against Enterococcus faecalis. 2014, 82 (11):4496-507 Infect. Immun.
Journal:
Infection and immunity
Issue Date:
Nov-2014
URI:
http://hdl.handle.net/10033/338595
DOI:
10.1128/IAI.02114-14
PubMed ID:
25114115
Type:
Article
Language:
en
ISSN:
1098-5522
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorScheb-Wetzel, Matthiasen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorBravo, Aliciaen
dc.contributor.authorGoldmann, Oliveren
dc.date.accessioned2015-01-20T13:24:43Z-
dc.date.available2015-01-20T13:24:43Z-
dc.date.issued2014-11-
dc.identifier.citationNew insights into the antimicrobial effect of mast cells against Enterococcus faecalis. 2014, 82 (11):4496-507 Infect. Immun.en
dc.identifier.issn1098-5522-
dc.identifier.pmid25114115-
dc.identifier.doi10.1128/IAI.02114-14-
dc.identifier.urihttp://hdl.handle.net/10033/338595-
dc.description.abstractEnterococcus faecalis has emerged as an important cause of life-threatening multidrug-resistant bacterial infections in the hospital setting. The pathogenesis of enterococcal infections has remained a relatively neglected field despite their obvious clinical relevance. The objective of this study was to characterize the interactions between mast cells (MCs), an innate immune cell population abundant in the intestinal lamina propria, and E. faecalis. This study was conducted with primary bone marrow-derived murine MCs. The results demonstrated that MCs exerted an antimicrobial effect against E. faecalis that was mediated both by degranulation, with the concomitant discharge of the antimicrobial effectors contained in the granules, and by the release of extracellular traps, in which E. faecalis was snared and killed. In particular, the cathelicidin LL-37 released by the MCs had potent antimicrobial effect against E. faecalis. We also investigated the specific receptors involved in the recognition of E. faecalis by MCs. We found that Toll-like receptors (TLRs) are critically involved in the MC recognition of E. faecalis, since MCs deficient in the expression of MyD88, an adaptor molecule required for signaling by most TLRs, were significantly impaired in their capacity to degranulate, to reduce E. faecalis growth as well as to release tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) after encountering this pathogen. Furthermore, TLR2 was identified as the most prominent TLR involved in the recognition of E. faecalis by MCs. The results of this study indicate that MCs may be important contributors to the host innate immune defenses against E. faecalis.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Adhesionen
dc.subject.meshBone Marrow Cellsen
dc.subject.meshCells, Cultureden
dc.subject.meshEnterococcus faecalisen
dc.subject.meshGram-Positive Bacterial Infectionsen
dc.subject.meshMast Cellsen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred C57BLen
dc.subject.meshMice, Knockouten
dc.subject.meshSignal Transductionen
dc.subject.meshSpecific Pathogen-Free Organismsen
dc.subject.meshToll-Like Receptor 2en
dc.titleNew insights into the antimicrobial effect of mast cells against Enterococcus faecalis.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection reseach,Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalInfection and immunityen
This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.