Influence of fenofibrate treatment on triacylglycerides, diacylglycerides and fatty acids in fructose fed rats.

2.50
Hdl Handle:
http://hdl.handle.net/10033/338621
Title:
Influence of fenofibrate treatment on triacylglycerides, diacylglycerides and fatty acids in fructose fed rats.
Authors:
Kopf, Thomas; Schaefer, Hans-Ludwig; Troetzmueller, Martin; Koefeler, Harald; Broenstrup, Mark; Konovalova, Tatiana; Schmitz, Gerd
Abstract:
Fenofibrate (FF) lowers plasma triglycerides via PPARα activation. Here, we analyzed lipidomic changes upon FF treatment of fructose fed rats. Three groups with 6 animals each were defined as control, fructose-fed and fructose-fed/FF treated. Male Wistar Unilever Rats were subjected to 10% fructose-feeding for 20 days. On day 14, fenofibrate treatment (100 mg/kg p.o.) was initiated and maintained for 7 days. Lipid species in serum were analyzed using mass spectrometry (ESI-MS/MS; LC-FT-MS, GC-MS) on days 0, 14 and 20 in all three groups. In addition, lipid levels in liver and intestine were determined. Short-chain TAGs increased in serum and liver upon fructose-feeding, while almost all TAG-species decreased under FF treatment. Long-chain unsaturated DAG-levels (36:1, 36:2, 36:4, 38:3, 38:4, 38:5) increased upon FF treatment in rat liver and decreased in rat serum. FAs, especially short-chain FAs (12:0, 14:0, 16:0) increased during fructose-challenge. VLDL secretion increased upon fructose-feeding and together with FA-levels decreased to control levels during FF treatment. Fructose challenge of de novo fatty acid synthesis through fatty acid synthase (FAS) may enhance the release of FAs ≤ 16:0 chain length, a process reversed by FF-mediated PPARα-activation.
Citation:
Influence of fenofibrate treatment on triacylglycerides, diacylglycerides and fatty acids in fructose fed rats. 2014, 9 (9):e106849 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/338621
DOI:
10.1371/journal.pone.0106849
PubMed ID:
25198467
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorKopf, Thomasen
dc.contributor.authorSchaefer, Hans-Ludwigen
dc.contributor.authorTroetzmueller, Martinen
dc.contributor.authorKoefeler, Haralden
dc.contributor.authorBroenstrup, Marken
dc.contributor.authorKonovalova, Tatianaen
dc.contributor.authorSchmitz, Gerden
dc.date.accessioned2015-01-22T11:57:04Z-
dc.date.available2015-01-22T11:57:04Z-
dc.date.issued2014-
dc.identifier.citationInfluence of fenofibrate treatment on triacylglycerides, diacylglycerides and fatty acids in fructose fed rats. 2014, 9 (9):e106849 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid25198467-
dc.identifier.doi10.1371/journal.pone.0106849-
dc.identifier.urihttp://hdl.handle.net/10033/338621-
dc.description.abstractFenofibrate (FF) lowers plasma triglycerides via PPARα activation. Here, we analyzed lipidomic changes upon FF treatment of fructose fed rats. Three groups with 6 animals each were defined as control, fructose-fed and fructose-fed/FF treated. Male Wistar Unilever Rats were subjected to 10% fructose-feeding for 20 days. On day 14, fenofibrate treatment (100 mg/kg p.o.) was initiated and maintained for 7 days. Lipid species in serum were analyzed using mass spectrometry (ESI-MS/MS; LC-FT-MS, GC-MS) on days 0, 14 and 20 in all three groups. In addition, lipid levels in liver and intestine were determined. Short-chain TAGs increased in serum and liver upon fructose-feeding, while almost all TAG-species decreased under FF treatment. Long-chain unsaturated DAG-levels (36:1, 36:2, 36:4, 38:3, 38:4, 38:5) increased upon FF treatment in rat liver and decreased in rat serum. FAs, especially short-chain FAs (12:0, 14:0, 16:0) increased during fructose-challenge. VLDL secretion increased upon fructose-feeding and together with FA-levels decreased to control levels during FF treatment. Fructose challenge of de novo fatty acid synthesis through fatty acid synthase (FAS) may enhance the release of FAs ≤ 16:0 chain length, a process reversed by FF-mediated PPARα-activation.en
dc.language.isoenen
dc.titleInfluence of fenofibrate treatment on triacylglycerides, diacylglycerides and fatty acids in fructose fed rats.en
dc.typeArticleen
dc.identifier.journalPloS oneen
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