2.50
Hdl Handle:
http://hdl.handle.net/10033/338659
Title:
Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.
Authors:
Kerckhof, Frederiek-Maarten; Courtens, Emilie N P; Geirnaert, Annelies; Hoefman, Sven; Ho, Adrian; Vilchez-Vargas, Ramiro; Pieper, Dietmar H; Jauregui, Ruy; Vlaeminck, Siegfried E; Van de Wiele, Tom; Vandamme, Peter; Heylen, Kim; Boon, Nico
Abstract:
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential applications for fecal transplants and pre/probiotics research. After three months of cryopreservation at -80 °C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. However, the activity of the fecal community was not influenced by the CPA addition, although the preservation of the community structure (as determined by 16S rRNA gene sequencing) was enhanced by addition of CPA. In summary, we have evaluated a cryopreservation protocol that succeeded in preserving both community structure and functionality of value-added microbiomes. This will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications.
Citation:
Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity. 2014, 9 (6):e99517 PLoS ONE
Journal:
PloS one
Issue Date:
2014
URI:
http://hdl.handle.net/10033/338659
DOI:
10.1371/journal.pone.0099517
PubMed ID:
24937032
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorKerckhof, Frederiek-Maartenen
dc.contributor.authorCourtens, Emilie N Pen
dc.contributor.authorGeirnaert, Anneliesen
dc.contributor.authorHoefman, Svenen
dc.contributor.authorHo, Adrianen
dc.contributor.authorVilchez-Vargas, Ramiroen
dc.contributor.authorPieper, Dietmar Hen
dc.contributor.authorJauregui, Ruyen
dc.contributor.authorVlaeminck, Siegfried Een
dc.contributor.authorVan de Wiele, Tomen
dc.contributor.authorVandamme, Peteren
dc.contributor.authorHeylen, Kimen
dc.contributor.authorBoon, Nicoen
dc.date.accessioned2015-01-22T10:20:31Z-
dc.date.available2015-01-22T10:20:31Z-
dc.date.issued2014-
dc.identifier.citationOptimized cryopreservation of mixed microbial communities for conserved functionality and diversity. 2014, 9 (6):e99517 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid24937032-
dc.identifier.doi10.1371/journal.pone.0099517-
dc.identifier.urihttp://hdl.handle.net/10033/338659-
dc.description.abstractThe use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential applications for fecal transplants and pre/probiotics research. After three months of cryopreservation at -80 °C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. However, the activity of the fecal community was not influenced by the CPA addition, although the preservation of the community structure (as determined by 16S rRNA gene sequencing) was enhanced by addition of CPA. In summary, we have evaluated a cryopreservation protocol that succeeded in preserving both community structure and functionality of value-added microbiomes. This will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications.en
dc.language.isoenen
dc.titleOptimized cryopreservation of mixed microbial communities for conserved functionality and diversity.en
dc.typeArticleen
dc.identifier.journalPloS oneen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.