2.50
Hdl Handle:
http://hdl.handle.net/10033/339034
Title:
Human bone marrow mesenchymal stem cells: a systematic reappraisal via the genostem experience.
Authors:
Charbord, Pierre; Livne, Erella; Gross, Gerhard; Häupl, Thomas; Neves, Nuno M; Marie, Pierre; Bianco, Paolo; Jorgensen, Christian
Abstract:
Genostem (acronym for "Adult mesenchymal stem cells engineering for connective tissue disorders. From the bench to the bed side") has been an European consortium of 30 teams working together on human bone marrow Mesenchymal Stem Cell (MSC) biological properties and repair capacity. Part of Genostem activity has been dedicated to the study of basic issues on undifferentiated MSCs properties and on signalling pathways leading to the differentiation into 3 of the connective tissue lineages, osteoblastic, chondrocytic and tenocytic. We have evidenced that native bone marrow MSCs and stromal cells, forming the niche of hematopoietic stem cells, were the same cellular entity located abluminally from marrow sinus endothelial cells. We have also shown that culture-amplified, clonogenic and highly-proliferative MSCs were bona fide stem cells, sharing with other stem cell types the major attributes of self-renewal and of multipotential priming to the lineages to which they can differentiate (osteoblasts, chondrocytes, adipocytes and vascular smooth muscle cells/pericytes). Extensive transcription profiling and in vitro and in vivo assays were applied to identify genes involved in differentiation. Thus we have described novel factors implicated in osteogenesis (FHL2, ITGA5, Fgf18), chondrogenesis (FOXO1A) and tenogenesis (Smad8). Another part of Genostem activity has been devoted to studies of the repair capacity of MSCs in animal models, a prerequisite for future clinical trials. We have developed novel scaffolds (chitosan, pharmacologically active microcarriers) useful for the repair of both bone and cartilage. Finally and most importantly, we have shown that locally implanted MSCs effectively repair bone, cartilage and tendon.
Citation:
Human bone marrow mesenchymal stem cells: a systematic reappraisal via the genostem experience. 2011, 7 (1):32-42 Stem Cell Rev
Journal:
Stem cell reviews
Issue Date:
Mar-2011
URI:
http://hdl.handle.net/10033/339034
DOI:
10.1007/s12015-010-9125-6
PubMed ID:
20198518
Type:
Article
Language:
en
ISSN:
1558-6804
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorCharbord, Pierreen
dc.contributor.authorLivne, Erellaen
dc.contributor.authorGross, Gerharden
dc.contributor.authorHäupl, Thomasen
dc.contributor.authorNeves, Nuno Men
dc.contributor.authorMarie, Pierreen
dc.contributor.authorBianco, Paoloen
dc.contributor.authorJorgensen, Christianen
dc.date.accessioned2015-01-29T12:08:13Z-
dc.date.available2015-01-29T12:08:13Z-
dc.date.issued2011-03-
dc.identifier.citationHuman bone marrow mesenchymal stem cells: a systematic reappraisal via the genostem experience. 2011, 7 (1):32-42 Stem Cell Reven
dc.identifier.issn1558-6804-
dc.identifier.pmid20198518-
dc.identifier.doi10.1007/s12015-010-9125-6-
dc.identifier.urihttp://hdl.handle.net/10033/339034-
dc.description.abstractGenostem (acronym for "Adult mesenchymal stem cells engineering for connective tissue disorders. From the bench to the bed side") has been an European consortium of 30 teams working together on human bone marrow Mesenchymal Stem Cell (MSC) biological properties and repair capacity. Part of Genostem activity has been dedicated to the study of basic issues on undifferentiated MSCs properties and on signalling pathways leading to the differentiation into 3 of the connective tissue lineages, osteoblastic, chondrocytic and tenocytic. We have evidenced that native bone marrow MSCs and stromal cells, forming the niche of hematopoietic stem cells, were the same cellular entity located abluminally from marrow sinus endothelial cells. We have also shown that culture-amplified, clonogenic and highly-proliferative MSCs were bona fide stem cells, sharing with other stem cell types the major attributes of self-renewal and of multipotential priming to the lineages to which they can differentiate (osteoblasts, chondrocytes, adipocytes and vascular smooth muscle cells/pericytes). Extensive transcription profiling and in vitro and in vivo assays were applied to identify genes involved in differentiation. Thus we have described novel factors implicated in osteogenesis (FHL2, ITGA5, Fgf18), chondrogenesis (FOXO1A) and tenogenesis (Smad8). Another part of Genostem activity has been devoted to studies of the repair capacity of MSCs in animal models, a prerequisite for future clinical trials. We have developed novel scaffolds (chitosan, pharmacologically active microcarriers) useful for the repair of both bone and cartilage. Finally and most importantly, we have shown that locally implanted MSCs effectively repair bone, cartilage and tendon.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBone Marrow Cellsen
dc.subject.meshCell Culture Techniquesen
dc.subject.meshCell Differentiationen
dc.subject.meshCell Proliferationen
dc.subject.meshHumansen
dc.subject.meshMesenchymal Stromal Cellsen
dc.subject.meshTissue Engineeringen
dc.titleHuman bone marrow mesenchymal stem cells: a systematic reappraisal via the genostem experience.en
dc.typeArticleen
dc.identifier.journalStem cell reviewsen
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