Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/344377
Title:
Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation.
Authors:
Mittal, Ankit; Schulze, Kai; Ebensen, Thomas ( 0000-0001-8906-063X ) ; Weißmann, Sebastian; Hansen, Steffi; Lehr, Claus Michael ( 0000-0002-5864-8462 ) ; Guzmán, Carlos A
Abstract:
Trans-follicular (TF) vaccination has recently been studied as a unique route for non-invasive transcutaneous vaccination. The present study aims to extensively characterize the immune responses triggered by TF vaccination using ovalbumin loaded chitosan-PLGA (poly lactic-co-glycolic acid) nanoparticles without skin pre-treatment to preserve skin integrity. The impact of formulation composition i.e. antigenic solution or antigen-loaded nanoparticles with or without adjuvant [bis-(3',5')-cyclic dimeric adenosine monophosphate] on immune response quality following TF immunization was analyzed and compared with immune responses obtained after tape stripping the skin. The results presented in this study confirm the ability of nanoparticle based vaccine formulations to deliver antigen across the intact skin via the follicular route, but at the same time demonstrate the necessity to include adjuvants to generate efficient antigen-specific humoral and cellular immune responses.
Affiliation:
Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
Citation:
Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation. 2015, 11 (1):147-54 Nanomedicine
Journal:
Nanomedicine : nanotechnology, biology, and medicine
Issue Date:
Jan-2015
URI:
http://hdl.handle.net/10033/344377
DOI:
10.1016/j.nano.2014.08.009
PubMed ID:
25200611
Type:
Article
Language:
en
ISSN:
1549-9642
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorMittal, Ankiten
dc.contributor.authorSchulze, Kaien
dc.contributor.authorEbensen, Thomasen
dc.contributor.authorWeißmann, Sebastianen
dc.contributor.authorHansen, Steffien
dc.contributor.authorLehr, Claus Michaelen
dc.contributor.authorGuzmán, Carlos Aen
dc.date.accessioned2015-02-11T15:49:31Zen
dc.date.available2015-02-11T15:49:31Zen
dc.date.issued2015-01en
dc.identifier.citationEfficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation. 2015, 11 (1):147-54 Nanomedicineen
dc.identifier.issn1549-9642en
dc.identifier.pmid25200611en
dc.identifier.doi10.1016/j.nano.2014.08.009en
dc.identifier.urihttp://hdl.handle.net/10033/344377en
dc.description.abstractTrans-follicular (TF) vaccination has recently been studied as a unique route for non-invasive transcutaneous vaccination. The present study aims to extensively characterize the immune responses triggered by TF vaccination using ovalbumin loaded chitosan-PLGA (poly lactic-co-glycolic acid) nanoparticles without skin pre-treatment to preserve skin integrity. The impact of formulation composition i.e. antigenic solution or antigen-loaded nanoparticles with or without adjuvant [bis-(3',5')-cyclic dimeric adenosine monophosphate] on immune response quality following TF immunization was analyzed and compared with immune responses obtained after tape stripping the skin. The results presented in this study confirm the ability of nanoparticle based vaccine formulations to deliver antigen across the intact skin via the follicular route, but at the same time demonstrate the necessity to include adjuvants to generate efficient antigen-specific humoral and cellular immune responses.en
dc.language.isoenen
dc.titleEfficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research (HZI), Braunschweig, Germany.en
dc.identifier.journalNanomedicine : nanotechnology, biology, and medicineen

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