2.50
Hdl Handle:
http://hdl.handle.net/10033/346484
Title:
Mechanisms of methods for hepatitis C virus inactivation.
Authors:
Pfaender, Stephanie; Brinkmann, Janine; Todt, Daniel; Riebesehl, Nina; Steinmann, Joerg; Steinmann, Jochen; Pietschmann, Thomas ( 0000-0001-5138-6239 ) ; Steinmann, Eike ( 0000-0002-8771-4262 )
Abstract:
Virus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and the influence of chemical disinfectants, heat, and UV treatment on HCV was analyzed by the determination of infectious particles in a limiting-dilution assay, by quantitative reverse transcription-PCR, by core enzyme-linked immunosorbent assay, and by proteolytic protection assay. All different inactivation methods resulted in a loss of HCV infectivity by targeting different parts of the virus particle. Alcohols such as ethanol and 2-propanol did not affect the viral RNA genome integrity but disrupted the viral envelope membrane in a capsid protection assay. Heat and UV treatment of HCV particles resulted in direct damage of the viral genome since transfection of viral particle-associated RNA into permissive cells did not initiate RNA replication. In addition, heat incubation at 80°C disrupted the HCV envelope, rendering the viral capsid susceptible to proteolytic digest. This study demonstrated the molecular processes of viral inactivation of an enveloped virus and should facilitate the development of effective disinfection strategies in infection control not only against HCV but also against other enveloped viruses.
Affiliation:
Institute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research,Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
Citation:
Mechanisms of methods for hepatitis C virus inactivation. 2015, 81 (5):1616-21 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
1-Mar-2015
URI:
http://hdl.handle.net/10033/346484
DOI:
10.1128/AEM.03580-14
PubMed ID:
25527548
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
publications of the research group virus transmission ([TC]VIRT)

Full metadata record

DC FieldValue Language
dc.contributor.authorPfaender, Stephanieen
dc.contributor.authorBrinkmann, Janineen
dc.contributor.authorTodt, Danielen
dc.contributor.authorRiebesehl, Ninaen
dc.contributor.authorSteinmann, Joergen
dc.contributor.authorSteinmann, Jochenen
dc.contributor.authorPietschmann, Thomasen
dc.contributor.authorSteinmann, Eikeen
dc.date.accessioned2015-03-10T16:11:55Zen
dc.date.available2015-03-10T16:11:55Zen
dc.date.issued2015-03-01en
dc.identifier.citationMechanisms of methods for hepatitis C virus inactivation. 2015, 81 (5):1616-21 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336en
dc.identifier.pmid25527548en
dc.identifier.doi10.1128/AEM.03580-14en
dc.identifier.urihttp://hdl.handle.net/10033/346484en
dc.description.abstractVirus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and the influence of chemical disinfectants, heat, and UV treatment on HCV was analyzed by the determination of infectious particles in a limiting-dilution assay, by quantitative reverse transcription-PCR, by core enzyme-linked immunosorbent assay, and by proteolytic protection assay. All different inactivation methods resulted in a loss of HCV infectivity by targeting different parts of the virus particle. Alcohols such as ethanol and 2-propanol did not affect the viral RNA genome integrity but disrupted the viral envelope membrane in a capsid protection assay. Heat and UV treatment of HCV particles resulted in direct damage of the viral genome since transfection of viral particle-associated RNA into permissive cells did not initiate RNA replication. In addition, heat incubation at 80°C disrupted the HCV envelope, rendering the viral capsid susceptible to proteolytic digest. This study demonstrated the molecular processes of viral inactivation of an enveloped virus and should facilitate the development of effective disinfection strategies in infection control not only against HCV but also against other enveloped viruses.en
dc.language.isoenen
dc.titleMechanisms of methods for hepatitis C virus inactivation.en
dc.typeArticleen
dc.contributor.departmentInstitute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research,Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalApplied and environmental microbiologyen

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