Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.

2.50
Hdl Handle:
http://hdl.handle.net/10033/347047
Title:
Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.
Authors:
Avican, Kemal; Fahlgren, Anna; Huss, Mikael; Heroven, Ann Kathrin; Beckstette, Michael; Dersch, Petra ( 0000-0001-8177-3280 ) ; Fällman, Maria
Abstract:
We recently found that Yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. We performed in vivo RNA-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. Comprehensive analysis of mixed RNA populations from infected tissues revealed that Y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of T3SS virulence genes during persistence when the pathogen resides within the cecum. At the persistent stage, the expression pattern in many respects resembles the pattern seen in vitro at 26oC, with for example, up-regulation of flagellar genes and invA. These findings are expected to have impact on future rationales to identify suitable bacterial targets for new antibiotics. Other genes that are up-regulated during persistence are genes involved in anaerobiosis, chemotaxis, and protection against oxidative and acidic stress, which indicates the influence of different environmental cues. We found that the Crp/CsrA/RovA regulatory cascades influence the pattern of bacterial gene expression during persistence. Furthermore, arcA, fnr, frdA, and wrbA play critical roles in persistence. Our findings suggest a model for the life cycle of this enteropathogen with reprogramming from a virulent to an adapted phenotype capable of persisting and spreading by fecal shedding.
Citation:
Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis. 2015, 11 (1):e1004600 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
Jan-2015
URI:
http://hdl.handle.net/10033/347047
DOI:
10.1371/journal.ppat.1004600
PubMed ID:
25590628
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorAvican, Kemalen
dc.contributor.authorFahlgren, Annaen
dc.contributor.authorHuss, Mikaelen
dc.contributor.authorHeroven, Ann Kathrinen
dc.contributor.authorBeckstette, Michaelen
dc.contributor.authorDersch, Petraen
dc.contributor.authorFällman, Mariaen
dc.date.accessioned2015-03-24T14:28:50Zen
dc.date.available2015-03-24T14:28:50Zen
dc.date.issued2015-01en
dc.identifier.citationReprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis. 2015, 11 (1):e1004600 PLoS Pathog.en
dc.identifier.issn1553-7374en
dc.identifier.pmid25590628en
dc.identifier.doi10.1371/journal.ppat.1004600en
dc.identifier.urihttp://hdl.handle.net/10033/347047en
dc.description.abstractWe recently found that Yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. We performed in vivo RNA-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. Comprehensive analysis of mixed RNA populations from infected tissues revealed that Y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of T3SS virulence genes during persistence when the pathogen resides within the cecum. At the persistent stage, the expression pattern in many respects resembles the pattern seen in vitro at 26oC, with for example, up-regulation of flagellar genes and invA. These findings are expected to have impact on future rationales to identify suitable bacterial targets for new antibiotics. Other genes that are up-regulated during persistence are genes involved in anaerobiosis, chemotaxis, and protection against oxidative and acidic stress, which indicates the influence of different environmental cues. We found that the Crp/CsrA/RovA regulatory cascades influence the pattern of bacterial gene expression during persistence. Furthermore, arcA, fnr, frdA, and wrbA play critical roles in persistence. Our findings suggest a model for the life cycle of this enteropathogen with reprogramming from a virulent to an adapted phenotype capable of persisting and spreading by fecal shedding.en
dc.language.isoenen
dc.titleReprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.en
dc.typeArticleen
dc.identifier.journalPLoS pathogensen

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