Genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products.

2.50
Hdl Handle:
http://hdl.handle.net/10033/528149
Title:
Genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products.
Authors:
Wang, Xiuna; Zhang, Xiaoling; Liu, Ling; Xiang, Meichun; Wang, Wenzhao; Sun, Xiang; Che, Yongsheng; Guo, Liangdong; Liu, Gang; Guo, Liyun; Wang, Chengshu; Yin, Wen-Bing; Stadler, Marc ( 0000-0002-7284-8671 ) ; Zhang, Xinyu; Liu, Xingzhong
Abstract:
BackgroundIn recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics.ResultsHere, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion.ConclusionThe significant expansion of pectinase encoding genes provides essential insights in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products. 2015, 16 (1):28 BMC Genomics
Journal:
BMC genomics
Issue Date:
27-Jan-2015
URI:
http://hdl.handle.net/10033/528149
DOI:
10.1186/s12864-014-1190-9
PubMed ID:
25623211
Type:
Article
ISSN:
1471-2164
Appears in Collections:
publications of the department of microbial drugs (MWIS)

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Xiunaen
dc.contributor.authorZhang, Xiaolingen
dc.contributor.authorLiu, Lingen
dc.contributor.authorXiang, Meichunen
dc.contributor.authorWang, Wenzhaoen
dc.contributor.authorSun, Xiangen
dc.contributor.authorChe, Yongshengen
dc.contributor.authorGuo, Liangdongen
dc.contributor.authorLiu, Gangen
dc.contributor.authorGuo, Liyunen
dc.contributor.authorWang, Chengshuen
dc.contributor.authorYin, Wen-Bingen
dc.contributor.authorStadler, Marcen
dc.contributor.authorZhang, Xinyuen
dc.contributor.authorLiu, Xingzhongen
dc.date.accessioned2015-04-10T14:11:42Zen
dc.date.available2015-04-10T14:11:42Zen
dc.date.issued2015-01-27en
dc.identifier.citationGenomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products. 2015, 16 (1):28 BMC Genomicsen
dc.identifier.issn1471-2164en
dc.identifier.pmid25623211en
dc.identifier.doi10.1186/s12864-014-1190-9en
dc.identifier.urihttp://hdl.handle.net/10033/528149en
dc.description.abstractBackgroundIn recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics.ResultsHere, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion.ConclusionThe significant expansion of pectinase encoding genes provides essential insights in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.en
dc.languageENGen
dc.titleGenomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBMC genomicsen

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