The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion.

2.50
Hdl Handle:
http://hdl.handle.net/10033/556662
Title:
The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion.
Authors:
Seele, Jana; Beineke, Andreas; Hillermann, Lena-Maria; Jaschok-Kentner, Beate; von Pawel-Rammingen, Ulrich; Valentin-Weigand, Peter; Baums, Christoph Georg
Abstract:
Streptococcus (S.) suis is one of the most important pathogens in pigs causing meningitis, arthritis, endocarditis and serositis. Furthermore, it is also an emerging zoonotic agent. In our previous work we identified a highly specific IgM protease in S. suis, designated Ide Ssuis . The objective of this study was to characterize the function of Ide Ssuis in the host-pathogen interaction. Edman-sequencing revealed that Ide Ssuis cleaves the heavy chain of the IgM molecule between constant domain 2 and 3. As the C1q binding motif is located in the C3 domain, we hypothesized that Ide Ssuis is involved in complement evasion. Complement-mediated hemolysis induced by porcine hyperimmune sera containing erythrocyte-specific IgM was abrogated by treatment of these sera with recombinant Ide Ssuis . Furthermore, expression of Ide Ssuis reduced IgM-triggered complement deposition on the bacterial surface. An infection experiment of prime-vaccinated growing piglets suggested attenuation in the virulence of the mutant 10Δide Ssuis . Bactericidal assays confirmed a positive effect of Ide Ssuis expression on bacterial survival in porcine blood in the presence of high titers of specific IgM. In conclusion, this study demonstrates that Ide Ssuis is a novel complement evasion factor, which is important for bacterial survival in porcine blood during the early adaptive (IgM-dominated) immune response.
Affiliation:
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Citation:
The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion. 2015, 46 (1):45 Vet. Res.
Journal:
Veterinary research
Issue Date:
2015
URI:
http://hdl.handle.net/10033/556662
DOI:
10.1186/s13567-015-0171-6
PubMed ID:
25928761
Type:
Article
Language:
en
ISSN:
1297-9716
Appears in Collections:
Publications of the Dept. Structure and Functions of Proteins(SFPR)

Full metadata record

DC FieldValue Language
dc.contributor.authorSeele, Janaen
dc.contributor.authorBeineke, Andreasen
dc.contributor.authorHillermann, Lena-Mariaen
dc.contributor.authorJaschok-Kentner, Beateen
dc.contributor.authorvon Pawel-Rammingen, Ulrichen
dc.contributor.authorValentin-Weigand, Peteren
dc.contributor.authorBaums, Christoph Georgen
dc.date.accessioned2015-06-10T14:13:04Zen
dc.date.available2015-06-10T14:13:04Zen
dc.date.issued2015en
dc.identifier.citationThe immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion. 2015, 46 (1):45 Vet. Res.en
dc.identifier.issn1297-9716en
dc.identifier.pmid25928761en
dc.identifier.doi10.1186/s13567-015-0171-6en
dc.identifier.urihttp://hdl.handle.net/10033/556662en
dc.description.abstractStreptococcus (S.) suis is one of the most important pathogens in pigs causing meningitis, arthritis, endocarditis and serositis. Furthermore, it is also an emerging zoonotic agent. In our previous work we identified a highly specific IgM protease in S. suis, designated Ide Ssuis . The objective of this study was to characterize the function of Ide Ssuis in the host-pathogen interaction. Edman-sequencing revealed that Ide Ssuis cleaves the heavy chain of the IgM molecule between constant domain 2 and 3. As the C1q binding motif is located in the C3 domain, we hypothesized that Ide Ssuis is involved in complement evasion. Complement-mediated hemolysis induced by porcine hyperimmune sera containing erythrocyte-specific IgM was abrogated by treatment of these sera with recombinant Ide Ssuis . Furthermore, expression of Ide Ssuis reduced IgM-triggered complement deposition on the bacterial surface. An infection experiment of prime-vaccinated growing piglets suggested attenuation in the virulence of the mutant 10Δide Ssuis . Bactericidal assays confirmed a positive effect of Ide Ssuis expression on bacterial survival in porcine blood in the presence of high titers of specific IgM. In conclusion, this study demonstrates that Ide Ssuis is a novel complement evasion factor, which is important for bacterial survival in porcine blood during the early adaptive (IgM-dominated) immune response.en
dc.language.isoenen
dc.relationeu-repo/grantAgreement/EC/FP7/281473en
dc.rightsopenAccessen_GB
dc.titleThe immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.en
dc.identifier.journalVeterinary researchen

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