2.50
Hdl Handle:
http://hdl.handle.net/10033/579499
Title:
Unveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw.
Authors:
Jiménez, Diego Javier; Chaves-Moreno, Diego; van Elsas, Jan Dirk
Abstract:
Based on the premise that plant biomass can be efficiently degraded by mixed microbial cultures and/or enzymes, we here applied a targeted metagenomics-based approach to explore the metabolic potential of two forest soil-derived lignocellulolytic microbial consortia, denoted RWS and TWS (bred on wheat straw). Using the metagenomes of three selected batches of two experimental systems, about 1.2 Gb of sequence was generated. Comparative analyses revealed an overrepresentation of predicted carbohydrate transporters (ABC, TonB and phosphotransferases), two-component sensing systems and β-glucosidases/galactosidases in the two consortia as compared to the forest soil inoculum. Additionally, "profiling" of carbohydrate-active enzymes showed significant enrichments of several genes encoding glycosyl hydrolases of families GH2, GH43, GH92 and GH95. Sequence analyses revealed these to be most strongly affiliated to genes present on the genomes of Sphingobacterium, Bacteroides, Flavobacterium and Pedobacter spp. Assembly of the RWS and TWS metagenomes generated 16,536 and 15,902 contigs of ≥10 Kb, respectively. Thirteen contigs, containing 39 glycosyl hydrolase genes, constitute novel (hemi)cellulose utilization loci with affiliation to sequences primarily found in the Bacteroidetes. Overall, this study provides deep insight in the plant polysaccharide degrading capabilities of microbial consortia bred from forest soil, highlighting their biotechnological potential.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
Unveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw. 2015, 5:13845 Sci Rep
Journal:
Scientific reports
Issue Date:
2015
URI:
http://hdl.handle.net/10033/579499
DOI:
10.1038/srep13845
PubMed ID:
26343383
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorJiménez, Diego Javieren
dc.contributor.authorChaves-Moreno, Diegoen
dc.contributor.authorvan Elsas, Jan Dirken
dc.date.accessioned2015-10-08T14:42:22Zen
dc.date.available2015-10-08T14:42:22Zen
dc.date.issued2015en
dc.identifier.citationUnveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw. 2015, 5:13845 Sci Repen
dc.identifier.issn2045-2322en
dc.identifier.pmid26343383en
dc.identifier.doi10.1038/srep13845en
dc.identifier.urihttp://hdl.handle.net/10033/579499en
dc.description.abstractBased on the premise that plant biomass can be efficiently degraded by mixed microbial cultures and/or enzymes, we here applied a targeted metagenomics-based approach to explore the metabolic potential of two forest soil-derived lignocellulolytic microbial consortia, denoted RWS and TWS (bred on wheat straw). Using the metagenomes of three selected batches of two experimental systems, about 1.2 Gb of sequence was generated. Comparative analyses revealed an overrepresentation of predicted carbohydrate transporters (ABC, TonB and phosphotransferases), two-component sensing systems and β-glucosidases/galactosidases in the two consortia as compared to the forest soil inoculum. Additionally, "profiling" of carbohydrate-active enzymes showed significant enrichments of several genes encoding glycosyl hydrolases of families GH2, GH43, GH92 and GH95. Sequence analyses revealed these to be most strongly affiliated to genes present on the genomes of Sphingobacterium, Bacteroides, Flavobacterium and Pedobacter spp. Assembly of the RWS and TWS metagenomes generated 16,536 and 15,902 contigs of ≥10 Kb, respectively. Thirteen contigs, containing 39 glycosyl hydrolase genes, constitute novel (hemi)cellulose utilization loci with affiliation to sequences primarily found in the Bacteroidetes. Overall, this study provides deep insight in the plant polysaccharide degrading capabilities of microbial consortia bred from forest soil, highlighting their biotechnological potential.en
dc.language.isoenen
dc.titleUnveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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