Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/580114
Title:
Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells.
Authors:
Bauer, Isabel; Günther, Juliane; Wheeler, Thomas T; Engelmann, Susanne; Seyfert, Hans-Martin
Abstract:
Considerably divergent data have been published from attempts to model the E. coli vs. S. aureus specific immune reaction of the udder using primary cultures of bovine mammary epithelial cells from cows (pbMEC). Some groups reported a swift, strong and transient inflammatory response against challenges with E. coli and only a weak and retarded response against S. aureus, in agreement with the respective reaction of the udder. Others found almost the reverse. Presence or absence of fetal calf serum distinguished the experimental setting between both groups. We examined here if this causes the divergent reaction of the pbMEC towards both pathogen species. We challenged pbMEC with proteins from heat killed E. coli or S. aureus pathogens or purified TLR2 and TLR4 ligands. The stimuli were applied in normal growth medium with (SM10) or without (SM0) 10% fetal calf serum, or in the basal medium supplemented with 10 mg/ml milk proteins (SM Milk).
Affiliation:
Helmholtz Center for Infection Research
Citation:
Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells. 2015, 11:172 BMC Vet. Res.
Journal:
BMC veterinary research
Issue Date:
2015
URI:
http://hdl.handle.net/10033/580114
DOI:
10.1186/s12917-015-0489-3
PubMed ID:
26219462
Type:
Article
Language:
en
ISSN:
1746-6148
Appears in Collections:
publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorBauer, Isabelen
dc.contributor.authorGünther, Julianeen
dc.contributor.authorWheeler, Thomas Ten
dc.contributor.authorEngelmann, Susanneen
dc.contributor.authorSeyfert, Hans-Martinen
dc.date.accessioned2015-10-22T11:23:36Zen
dc.date.available2015-10-22T11:23:36Zen
dc.date.issued2015en
dc.identifier.citationExtracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells. 2015, 11:172 BMC Vet. Res.en
dc.identifier.issn1746-6148en
dc.identifier.pmid26219462en
dc.identifier.doi10.1186/s12917-015-0489-3en
dc.identifier.urihttp://hdl.handle.net/10033/580114en
dc.description.abstractConsiderably divergent data have been published from attempts to model the E. coli vs. S. aureus specific immune reaction of the udder using primary cultures of bovine mammary epithelial cells from cows (pbMEC). Some groups reported a swift, strong and transient inflammatory response against challenges with E. coli and only a weak and retarded response against S. aureus, in agreement with the respective reaction of the udder. Others found almost the reverse. Presence or absence of fetal calf serum distinguished the experimental setting between both groups. We examined here if this causes the divergent reaction of the pbMEC towards both pathogen species. We challenged pbMEC with proteins from heat killed E. coli or S. aureus pathogens or purified TLR2 and TLR4 ligands. The stimuli were applied in normal growth medium with (SM10) or without (SM0) 10% fetal calf serum, or in the basal medium supplemented with 10 mg/ml milk proteins (SM Milk).en
dc.language.isoenen
dc.titleExtracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Center for Infection Researchen
dc.identifier.journalBMC veterinary researchen

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