2.50
Hdl Handle:
http://hdl.handle.net/10033/581077
Title:
Anaerobic naphthalene degradation by sulfate-reducing Desulfobacteraceae from various anoxic aquifers.
Authors:
Kümmel, Steffen; Herbst, Florian-Alexander; Bahr, Arne; Duarte, Márcia; Pieper, Dietmar H; Jehmlich, Nico; Seifert, Jana; von Bergen, Martin; Bombach, Petra; Richnow, Hans H; Vogt, Carsten ( 0000-0002-7605-6356 )
Abstract:
Polycyclic aromatic hydrocarbons (PAH) are widespread and persistent environmental contaminants, especially in oxygen-free environments. The occurrence of anaerobic PAH-degrading bacteria and their underlying metabolic pathways are rarely known. In this study, PAH degraders were enriched in laboratory microcosms under sulfate-reducing conditions using groundwater and sediment samples from four PAH-contaminated aquifers. Five enrichment cultures were obtained showing sulfate-dependent naphthalene degradation. Mineralization of naphthalene was demonstrated by the formation of sulfide concomitant with the depletion of naphthalene and the development of (13)C-labeled CO2 from [(13)C6]-naphthalene. 16S rRNA gene and metaproteome analyses revealed that organisms related to Desulfobacterium str. N47 were the main naphthalene degraders in four enrichment cultures. Protein sequences highly similar to enzymes of the naphthalene degradation pathway of N47 were identified, suggesting that naphthalene was activated by a carboxylase, and that the central metabolite 2-naphthoyl-CoA was further reduced by two reductases. The data indicate an importance of members of the family Desulfobacteraceae for naphthalene degradation under sulfate-reducing conditions in freshwater environments.
Affiliation:
UFZ - Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstraße 15, Leipzig, Germany.
Citation:
Anaerobic naphthalene degradation by sulfate-reducing Desulfobacteraceae from various anoxic aquifers. 2015, 91 (3): FEMS Microbiol. Ecol.
Journal:
FEMS microbiology ecology
Issue Date:
Mar-2015
URI:
http://hdl.handle.net/10033/581077
DOI:
10.1093/femsec/fiv006
PubMed ID:
25764566
Type:
Article
Language:
en
ISSN:
1574-6941
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorKümmel, Steffenen
dc.contributor.authorHerbst, Florian-Alexanderen
dc.contributor.authorBahr, Arneen
dc.contributor.authorDuarte, Márciaen
dc.contributor.authorPieper, Dietmar Hen
dc.contributor.authorJehmlich, Nicoen
dc.contributor.authorSeifert, Janaen
dc.contributor.authorvon Bergen, Martinen
dc.contributor.authorBombach, Petraen
dc.contributor.authorRichnow, Hans Hen
dc.contributor.authorVogt, Carstenen
dc.date.accessioned2015-10-26T10:21:23Zen
dc.date.available2015-10-26T10:21:23Zen
dc.date.issued2015-03en
dc.identifier.citationAnaerobic naphthalene degradation by sulfate-reducing Desulfobacteraceae from various anoxic aquifers. 2015, 91 (3): FEMS Microbiol. Ecol.en
dc.identifier.issn1574-6941en
dc.identifier.pmid25764566en
dc.identifier.doi10.1093/femsec/fiv006en
dc.identifier.urihttp://hdl.handle.net/10033/581077en
dc.description.abstractPolycyclic aromatic hydrocarbons (PAH) are widespread and persistent environmental contaminants, especially in oxygen-free environments. The occurrence of anaerobic PAH-degrading bacteria and their underlying metabolic pathways are rarely known. In this study, PAH degraders were enriched in laboratory microcosms under sulfate-reducing conditions using groundwater and sediment samples from four PAH-contaminated aquifers. Five enrichment cultures were obtained showing sulfate-dependent naphthalene degradation. Mineralization of naphthalene was demonstrated by the formation of sulfide concomitant with the depletion of naphthalene and the development of (13)C-labeled CO2 from [(13)C6]-naphthalene. 16S rRNA gene and metaproteome analyses revealed that organisms related to Desulfobacterium str. N47 were the main naphthalene degraders in four enrichment cultures. Protein sequences highly similar to enzymes of the naphthalene degradation pathway of N47 were identified, suggesting that naphthalene was activated by a carboxylase, and that the central metabolite 2-naphthoyl-CoA was further reduced by two reductases. The data indicate an importance of members of the family Desulfobacteraceae for naphthalene degradation under sulfate-reducing conditions in freshwater environments.en
dc.language.isoenen
dc.subject.meshAnaerobiosisen
dc.subject.meshCarboxy-Lyasesen
dc.subject.meshDeltaproteobacteriaen
dc.subject.meshEnvironmental Pollutantsen
dc.subject.meshEnvironmental Restoration and Remediationen
dc.subject.meshGroundwateren
dc.subject.meshNaphthalenesen
dc.subject.meshOxidation-Reductionen
dc.subject.meshOxidoreductasesen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSulfatesen
dc.titleAnaerobic naphthalene degradation by sulfate-reducing Desulfobacteraceae from various anoxic aquifers.en
dc.typeArticleen
dc.contributor.departmentUFZ - Helmholtz Centre for Environmental Research, Department of Isotope Biogeochemistry, Permoserstraße 15, Leipzig, Germany.en
dc.identifier.journalFEMS microbiology ecologyen

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