Microbial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway.

2.50
Hdl Handle:
http://hdl.handle.net/10033/581120
Title:
Microbial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway.
Authors:
Martínez-Lavanchy, P M; Chen, Z; Lünsmann, V; Marin-Cevada, V; Vilchez-Vargas, R ( 0000-0002-7220-258X ) ; Pieper, D H; Reiche, N; Kappelmeyer, U; Imparato, V; Junca, H ( 0000-0003-4546-6229 ) ; Nijenhuis, I; Müller, J A; Kuschk, P; Heipieper, H J
Abstract:
In the present study, microbial toluene degradation in controlled constructed wetland model systems, planted fixed-bed reactors (PFRs), was queried with DNA-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. Two PFR replicates were operated with toluene as the sole external carbon and electron source for 2 years. The bulk redox conditions in these systems were hypoxic to anoxic. The autochthonous bacterial communities, as analyzed by Illumina sequencing of 16S rRNA gene amplicons, were mainly comprised of the families Xanthomonadaceae, Comamonadaceae, and Burkholderiaceae, plus Rhodospirillaceae in one of the PFR replicates. DNA microarray analyses of the catabolic potentials for aromatic compound degradation suggested the presence of the ring monooxygenation pathway in both systems, as well as the anaerobic toluene pathway in the PFR replicate with a high abundance of Rhodospirillaceae. The presence of catabolic genes encoding the ring monooxygenation pathway was verified by quantitative PCR analysis, utilizing the obtained toluene-degrading isolates as references. Stable isotope fractionation analysis showed low-level of carbon fractionation and only minimal hydrogen fractionation in both PFRs, which matches the fractionation signatures of monooxygenation and dioxygenation. In combination with the results of the DNA-based analyses, this suggests that toluene degradation occurs predominantly via ring monooxygenation in the PFRs.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
Microbial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway. 2015, 81 (18):6241-52 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
15-Sep-2015
URI:
http://hdl.handle.net/10033/581120
DOI:
10.1128/AEM.01822-15
PubMed ID:
26150458
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorMartínez-Lavanchy, P Men
dc.contributor.authorChen, Zen
dc.contributor.authorLünsmann, Ven
dc.contributor.authorMarin-Cevada, Ven
dc.contributor.authorVilchez-Vargas, Ren
dc.contributor.authorPieper, D Hen
dc.contributor.authorReiche, Nen
dc.contributor.authorKappelmeyer, Uen
dc.contributor.authorImparato, Ven
dc.contributor.authorJunca, Hen
dc.contributor.authorNijenhuis, Ien
dc.contributor.authorMüller, J Aen
dc.contributor.authorKuschk, Pen
dc.contributor.authorHeipieper, H Jen
dc.date.accessioned2015-10-26T15:09:15Zen
dc.date.available2015-10-26T15:09:15Zen
dc.date.issued2015-09-15en
dc.identifier.citationMicrobial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway. 2015, 81 (18):6241-52 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336en
dc.identifier.pmid26150458en
dc.identifier.doi10.1128/AEM.01822-15en
dc.identifier.urihttp://hdl.handle.net/10033/581120en
dc.description.abstractIn the present study, microbial toluene degradation in controlled constructed wetland model systems, planted fixed-bed reactors (PFRs), was queried with DNA-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. Two PFR replicates were operated with toluene as the sole external carbon and electron source for 2 years. The bulk redox conditions in these systems were hypoxic to anoxic. The autochthonous bacterial communities, as analyzed by Illumina sequencing of 16S rRNA gene amplicons, were mainly comprised of the families Xanthomonadaceae, Comamonadaceae, and Burkholderiaceae, plus Rhodospirillaceae in one of the PFR replicates. DNA microarray analyses of the catabolic potentials for aromatic compound degradation suggested the presence of the ring monooxygenation pathway in both systems, as well as the anaerobic toluene pathway in the PFR replicate with a high abundance of Rhodospirillaceae. The presence of catabolic genes encoding the ring monooxygenation pathway was verified by quantitative PCR analysis, utilizing the obtained toluene-degrading isolates as references. Stable isotope fractionation analysis showed low-level of carbon fractionation and only minimal hydrogen fractionation in both PFRs, which matches the fractionation signatures of monooxygenation and dioxygenation. In combination with the results of the DNA-based analyses, this suggests that toluene degradation occurs predominantly via ring monooxygenation in the PFRs.en
dc.language.isoenen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/211684en
dc.rightsopenAccessen
dc.titleMicrobial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalApplied and environmental microbiologyen

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