Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.

2.50
Hdl Handle:
http://hdl.handle.net/10033/594409
Title:
Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.
Authors:
Dammeyer, Thorben; Steinwand, Miriam; Krüger, Sarah-C; Dübel, Stefan; Hust, Michael; Timmis, Kenneth N
Abstract:
Recombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 2011, 10:11 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2011
URI:
http://hdl.handle.net/10033/594409
DOI:
10.1186/1475-2859-10-11
PubMed ID:
21338491
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValue Language
dc.contributor.authorDammeyer, Thorbenen
dc.contributor.authorSteinwand, Miriamen
dc.contributor.authorKrüger, Sarah-Cen
dc.contributor.authorDübel, Stefanen
dc.contributor.authorHust, Michaelen
dc.contributor.authorTimmis, Kenneth Nen
dc.date.accessioned2016-01-20T14:30:25Zen
dc.date.available2016-01-20T14:30:25Zen
dc.date.issued2011en
dc.identifier.citationEfficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 2011, 10:11 Microb. Cell Fact.en
dc.identifier.issn1475-2859en
dc.identifier.pmid21338491en
dc.identifier.doi10.1186/1475-2859-10-11en
dc.identifier.urihttp://hdl.handle.net/10033/594409en
dc.description.abstractRecombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshC-Reactive Proteinen
dc.subject.meshHumansen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMucin-1en
dc.subject.meshPeriplasmen
dc.subject.meshProtein Bindingen
dc.subject.meshPseudomonas putidaen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshSingle-Chain Antibodiesen
dc.subject.meshSolubilityen
dc.titleEfficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen
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