2.50
Hdl Handle:
http://hdl.handle.net/10033/595959
Title:
Bacteriomimetic invasin-functionalized nanocarriers for intracellular delivery.
Authors:
Labouta, Hagar Ibrahim; Menina, Sara; Kochut, Annika; Gordon, Sarah; Geyer, Rebecca; Dersch, Petra; Lehr, Claus-Michael
Abstract:
Intracellular bacteria invade mammalian cells to establish an infectious niche. The current work models adhesion and subsequent internalization strategy of pathogenic bacteria into mammalian cells to design a bacteriomimetic bioinvasive delivery system. We report on the surface functionalization of liposomes with a C-terminal fragment of invasin (InvA497), an invasion factor in the outer membrane of Yersinia pseudotuberculosis. InvA497-functionalized liposomes adhere to mammalian epithelial HEp-2 cell line at different infection stages with a significantly higher efficiency than liposomes functionalized with bovine serum albumin. Covalent attachment of InvA497 results in higher cellular adhesion than liposomes with physically adsorbed InvA497 with non-specific surface protein alignment. Uptake studies in HEp-2 cells indicate active internalization of InvA497-functionalized liposomes via β1-integrin receptor-mediated uptake mechanism mimicking the natural invasion strategy of Y. pseudotuberculosis. Uptake studies in Caco-2 cells at different polarization states demonstrate specific targeting of the InvA497-functionalized liposomes to less polarized cells reflecting the status of inflamed cells. Moreover, when loaded with the anti-infective agent gentamicin and applied to HEp-2 cells infected with Y. pseudotuberculosis, InvA497-functionalized liposomes are able to significantly reduce the infection load relative to non-functionalized drug-loaded liposomes. This indicates a promising application of such a bacteriomimetic system for drug delivery to intracellular compartments.
Affiliation:
Helmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.
Citation:
Bacteriomimetic invasin-functionalized nanocarriers for intracellular delivery. 2015, 220 (Pt A):414-24 J Control Release
Journal:
Journal of controlled release : official journal of the Controlled Release Society
Issue Date:
28-Dec-2015
URI:
http://hdl.handle.net/10033/595959
DOI:
10.1016/j.jconrel.2015.10.052
PubMed ID:
26522071
Type:
Article
Language:
en
ISSN:
1873-4995
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorLabouta, Hagar Ibrahimen
dc.contributor.authorMenina, Saraen
dc.contributor.authorKochut, Annikaen
dc.contributor.authorGordon, Sarahen
dc.contributor.authorGeyer, Rebeccaen
dc.contributor.authorDersch, Petraen
dc.contributor.authorLehr, Claus-Michaelen
dc.date.accessioned2016-02-09T13:56:58Zen
dc.date.available2016-02-09T13:56:58Zen
dc.date.issued2015-12-28en
dc.identifier.citationBacteriomimetic invasin-functionalized nanocarriers for intracellular delivery. 2015, 220 (Pt A):414-24 J Control Releaseen
dc.identifier.issn1873-4995en
dc.identifier.pmid26522071en
dc.identifier.doi10.1016/j.jconrel.2015.10.052en
dc.identifier.urihttp://hdl.handle.net/10033/595959en
dc.description.abstractIntracellular bacteria invade mammalian cells to establish an infectious niche. The current work models adhesion and subsequent internalization strategy of pathogenic bacteria into mammalian cells to design a bacteriomimetic bioinvasive delivery system. We report on the surface functionalization of liposomes with a C-terminal fragment of invasin (InvA497), an invasion factor in the outer membrane of Yersinia pseudotuberculosis. InvA497-functionalized liposomes adhere to mammalian epithelial HEp-2 cell line at different infection stages with a significantly higher efficiency than liposomes functionalized with bovine serum albumin. Covalent attachment of InvA497 results in higher cellular adhesion than liposomes with physically adsorbed InvA497 with non-specific surface protein alignment. Uptake studies in HEp-2 cells indicate active internalization of InvA497-functionalized liposomes via β1-integrin receptor-mediated uptake mechanism mimicking the natural invasion strategy of Y. pseudotuberculosis. Uptake studies in Caco-2 cells at different polarization states demonstrate specific targeting of the InvA497-functionalized liposomes to less polarized cells reflecting the status of inflamed cells. Moreover, when loaded with the anti-infective agent gentamicin and applied to HEp-2 cells infected with Y. pseudotuberculosis, InvA497-functionalized liposomes are able to significantly reduce the infection load relative to non-functionalized drug-loaded liposomes. This indicates a promising application of such a bacteriomimetic system for drug delivery to intracellular compartments.en
dc.language.isoenen
dc.titleBacteriomimetic invasin-functionalized nanocarriers for intracellular delivery.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.en
dc.identifier.journalJournal of controlled release : official journal of the Controlled Release Societyen

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