2.50
Hdl Handle:
http://hdl.handle.net/10033/596967
Title:
Surface-modified yeast cells: A novel eukaryotic carrier for oral application.
Authors:
Kenngott, Elisabeth E; Kiefer, Ruth; Schneider-Daum, Nicole; Hamann, Alf; Schneider, Marc ( 0000-0002-9260-7357 ) ; Schmitt, Manfred J; Breinig, Frank
Abstract:
The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.
Affiliation:
Helmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.
Citation:
Surface-modified yeast cells: A novel eukaryotic carrier for oral application. 2016, 224:1-7 J Control Release
Journal:
Journal of controlled release : official journal of the Controlled Release Society
Issue Date:
28-Feb-2016
URI:
http://hdl.handle.net/10033/596967
DOI:
10.1016/j.jconrel.2015.12.054
PubMed ID:
26763373
Type:
Article
Language:
en
ISSN:
1873-4995
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorKenngott, Elisabeth Een
dc.contributor.authorKiefer, Ruthen
dc.contributor.authorSchneider-Daum, Nicoleen
dc.contributor.authorHamann, Alfen
dc.contributor.authorSchneider, Marcen
dc.contributor.authorSchmitt, Manfred Jen
dc.contributor.authorBreinig, Franken
dc.date.accessioned2016-02-23T14:20:57Zen
dc.date.available2016-02-23T14:20:57Zen
dc.date.issued2016-02-28en
dc.identifier.citationSurface-modified yeast cells: A novel eukaryotic carrier for oral application. 2016, 224:1-7 J Control Releaseen
dc.identifier.issn1873-4995en
dc.identifier.pmid26763373en
dc.identifier.doi10.1016/j.jconrel.2015.12.054en
dc.identifier.urihttp://hdl.handle.net/10033/596967en
dc.description.abstractThe effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.en
dc.language.isoenen
dc.titleSurface-modified yeast cells: A novel eukaryotic carrier for oral application.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.en
dc.identifier.journalJournal of controlled release : official journal of the Controlled Release Societyen

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