Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.

2.50
Hdl Handle:
http://hdl.handle.net/10033/607739
Title:
Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.
Authors:
Thiam, Hawa-Racine; Vargas, Pablo; Carpi, Nicolas; Crespo, Carolina Lage; Raab, Matthew; Terriac, Emmanuel; King, Megan C; Jacobelli, Jordan; Alberts, Arthur S; Stradal, Theresia; Lennon-Dumenil, Ana-Maria; Piel, Matthieu
Abstract:
Cell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells' requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. 2016, 7:10997 Nat Commun
Journal:
Nature communications
Issue Date:
2016
URI:
http://hdl.handle.net/10033/607739
DOI:
10.1038/ncomms10997
PubMed ID:
26975831
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
publications of the department of cell biology (ZBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorThiam, Hawa-Racineen
dc.contributor.authorVargas, Pabloen
dc.contributor.authorCarpi, Nicolasen
dc.contributor.authorCrespo, Carolina Lageen
dc.contributor.authorRaab, Matthewen
dc.contributor.authorTerriac, Emmanuelen
dc.contributor.authorKing, Megan Cen
dc.contributor.authorJacobelli, Jordanen
dc.contributor.authorAlberts, Arthur Sen
dc.contributor.authorStradal, Theresiaen
dc.contributor.authorLennon-Dumenil, Ana-Mariaen
dc.contributor.authorPiel, Matthieuen
dc.date.accessioned2016-05-03T13:57:47Zen
dc.date.available2016-05-03T13:57:47Zen
dc.date.issued2016en
dc.identifier.citationPerinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. 2016, 7:10997 Nat Communen
dc.identifier.issn2041-1723en
dc.identifier.pmid26975831en
dc.identifier.doi10.1038/ncomms10997en
dc.identifier.urihttp://hdl.handle.net/10033/607739en
dc.description.abstractCell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells' requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.en
dc.language.isoenen
dc.titlePerinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalNature communicationsen
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