2.50
Hdl Handle:
http://hdl.handle.net/10033/609272
Title:
Global antibody response to Staphylococcus aureus live-cell vaccination.
Authors:
Selle, Martina; Hertlein, Tobias; Oesterreich, Babett; Klemm, Theresa; Kloppot, Peggy; Müller, Elke; Ehricht, Ralf; Stentzel, Sebastian; Bröker, Barbara M; Engelmann, Susanne; Ohlsen, Knut
Abstract:
The pathogen Staphylococcus aureus causes a broad range of severe diseases and is feared for its ability to rapidly develop resistance to antibiotic substances. The increasing number of highly resistant S. aureus infections has accelerated the search for alternative treatment options to close the widening gap in anti-S. aureus therapy. This study analyses the humoral immune response to vaccination of Balb/c mice with sublethal doses of live S. aureus. The elicited antibody pattern in the sera of intravenously and intramuscularly vaccinated mice was determined using of a recently developed protein array. We observed a specific antibody response against a broad set of S. aureus antigens which was stronger following i.v. than i.m. vaccination. Intravenous but not intramuscular vaccination protected mice against an intramuscular challenge infection with a high bacterial dose. Vaccine protection was correlated with the strength of the anti-S. aureus antibody response. This study identified novel vaccine candidates by using protein microarrays as an effective tool and showed that successful vaccination against S. aureus relies on the optimal route of administration.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Global antibody response to Staphylococcus aureus live-cell vaccination. 2016, 6:24754 Sci Rep
Journal:
Scientific reports
Issue Date:
2016
URI:
http://hdl.handle.net/10033/609272
DOI:
10.1038/srep24754
PubMed ID:
27103319
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorSelle, Martinaen
dc.contributor.authorHertlein, Tobiasen
dc.contributor.authorOesterreich, Babetten
dc.contributor.authorKlemm, Theresaen
dc.contributor.authorKloppot, Peggyen
dc.contributor.authorMüller, Elkeen
dc.contributor.authorEhricht, Ralfen
dc.contributor.authorStentzel, Sebastianen
dc.contributor.authorBröker, Barbara Men
dc.contributor.authorEngelmann, Susanneen
dc.contributor.authorOhlsen, Knuten
dc.date.accessioned2016-05-13T14:08:25Zen
dc.date.available2016-05-13T14:08:25Zen
dc.date.issued2016en
dc.identifier.citationGlobal antibody response to Staphylococcus aureus live-cell vaccination. 2016, 6:24754 Sci Repen
dc.identifier.issn2045-2322en
dc.identifier.pmid27103319en
dc.identifier.doi10.1038/srep24754en
dc.identifier.urihttp://hdl.handle.net/10033/609272en
dc.description.abstractThe pathogen Staphylococcus aureus causes a broad range of severe diseases and is feared for its ability to rapidly develop resistance to antibiotic substances. The increasing number of highly resistant S. aureus infections has accelerated the search for alternative treatment options to close the widening gap in anti-S. aureus therapy. This study analyses the humoral immune response to vaccination of Balb/c mice with sublethal doses of live S. aureus. The elicited antibody pattern in the sera of intravenously and intramuscularly vaccinated mice was determined using of a recently developed protein array. We observed a specific antibody response against a broad set of S. aureus antigens which was stronger following i.v. than i.m. vaccination. Intravenous but not intramuscular vaccination protected mice against an intramuscular challenge infection with a high bacterial dose. Vaccine protection was correlated with the strength of the anti-S. aureus antibody response. This study identified novel vaccine candidates by using protein microarrays as an effective tool and showed that successful vaccination against S. aureus relies on the optimal route of administration.en
dc.language.isoenen
dc.titleGlobal antibody response to Staphylococcus aureus live-cell vaccination.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen
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