Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release.

2.50
Hdl Handle:
http://hdl.handle.net/10033/611365
Title:
Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release.
Authors:
Rietscher, René; Czaplewska, Justyna A; Majdanski, Tobias C; Gottschaldt, Michael; Schubert, Ulrich S; Schneider, Marc ( 0000-0002-9260-7357 ) ; Lehr, Claus-Michael
Abstract:
The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. In contrast, incorporating a PAGE block between the PEG and PLGA units, also eventually enabling active targeting introducing a reactive group, led to a significantly higher loading (+25%) and release rate (+100%), compared to PLGA and PEG-b-PLGA NPs.
Citation:
Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release. 2016, 500 (1-2):187-95 Int J Pharm
Journal:
International journal of pharmaceutics
Issue Date:
16-Mar-2016
URI:
http://hdl.handle.net/10033/611365
DOI:
10.1016/j.ijpharm.2016.01.021
PubMed ID:
26784983
Type:
Article
Language:
en
ISSN:
1873-3476
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorRietscher, Renéen
dc.contributor.authorCzaplewska, Justyna Aen
dc.contributor.authorMajdanski, Tobias Cen
dc.contributor.authorGottschaldt, Michaelen
dc.contributor.authorSchubert, Ulrich Sen
dc.contributor.authorSchneider, Marcen
dc.contributor.authorLehr, Claus-Michaelen
dc.date.accessioned2016-06-01T09:55:59Zen
dc.date.available2016-06-01T09:55:59Zen
dc.date.issued2016-03-16en
dc.identifier.citationImpact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release. 2016, 500 (1-2):187-95 Int J Pharmen
dc.identifier.issn1873-3476en
dc.identifier.pmid26784983en
dc.identifier.doi10.1016/j.ijpharm.2016.01.021en
dc.identifier.urihttp://hdl.handle.net/10033/611365en
dc.description.abstractThe effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. In contrast, incorporating a PAGE block between the PEG and PLGA units, also eventually enabling active targeting introducing a reactive group, led to a significantly higher loading (+25%) and release rate (+100%), compared to PLGA and PEG-b-PLGA NPs.en
dc.language.isoenen
dc.titleImpact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release.en
dc.typeArticleen
dc.identifier.journalInternational journal of pharmaceuticsen

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