A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.

2.50
Hdl Handle:
http://hdl.handle.net/10033/611701
Title:
A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.
Authors:
Kashani, Elham; Föhse, Lisa; Raha, Solaiman; Sandrock, Inga; Oberdörfer, Linda; Koenecke, Christian; Suerbaum, Sebastian; Weiss, Siegfried; Prinz, Immo
Abstract:
Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. 2015, 6:6477 Nat Commun
Journal:
Nature communications
Issue Date:
2015
URI:
http://hdl.handle.net/10033/611701
DOI:
10.1038/ncomms7477
PubMed ID:
25765849
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorKashani, Elhamen
dc.contributor.authorFöhse, Lisaen
dc.contributor.authorRaha, Solaimanen
dc.contributor.authorSandrock, Ingaen
dc.contributor.authorOberdörfer, Lindaen
dc.contributor.authorKoenecke, Christianen
dc.contributor.authorSuerbaum, Sebastianen
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authorPrinz, Immoen
dc.date.accessioned2016-06-03T10:28:26Zen
dc.date.available2016-06-03T10:28:26Zen
dc.date.issued2015en
dc.identifier.citationA clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. 2015, 6:6477 Nat Communen
dc.identifier.issn2041-1723en
dc.identifier.pmid25765849en
dc.identifier.doi10.1038/ncomms7477en
dc.identifier.urihttp://hdl.handle.net/10033/611701en
dc.description.abstractHere we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.en
dc.language.isoenen
dc.subject.meshAmino Acid Motifsen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshAnimalsen
dc.subject.meshAntigens, CD27en
dc.subject.meshCell Movementen
dc.subject.meshGerm-Line Mutationen
dc.subject.meshGreen Fluorescent Proteinsen
dc.subject.meshImmunity, Innateen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred C57BLen
dc.subject.meshMice, Transgenicen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPhenotypeen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshReceptors, Antigen, T-Cell, gamma-deltaen
dc.subject.meshReceptors, CCR6en
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshSingle-Cell Analysisen
dc.subject.meshT-Lymphocyte Subsetsen
dc.subject.meshThymus Glanden
dc.subject.meshTissue Distributionen
dc.titleA clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalNature communicationsen

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