2.50
Hdl Handle:
http://hdl.handle.net/10033/612299
Title:
Planctomycetes do possess a peptidoglycan cell wall.
Authors:
Jeske, Olga; Schüler, Margarete; Schumann, Peter; Schneider, Alexander; Boedeker, Christian; Jogler, Mareike; Bollschweiler, Daniel; Rohde, Manfred; Mayer, Christoph; Engelhardt, Harald; Spring, Stefan; Jogler, Christian
Abstract:
Most bacteria contain a peptidoglycan (PG) cell wall, which is critical for maintenance of shape and important for cell division. In contrast, Planctomycetes have been proposed to produce a proteinaceous cell wall devoid of PG. The apparent absence of PG has been used as an argument for the putative planctomycetal ancestry of all bacterial lineages. Here we show, employing multiple bioinformatic methods, that planctomycetal genomes encode proteins required for PG synthesis. Furthermore, we biochemically demonstrate the presence of the sugar and the peptide components of PG in Planctomycetes. In addition, light and electron microscopic experiments reveal planctomycetal PG sacculi that are susceptible to lysozyme treatment. Finally, cryo-electron tomography demonstrates that Planctomycetes possess a typical PG cell wall and that their cellular architecture is thus more similar to that of other Gram-negative bacteria. Our findings shed new light on the cellular architecture and cell division of the maverick Planctomycetes.
Affiliation:
Helmholtzzentrum für Infektionsforschung 38124 Braunschweig
Citation:
Planctomycetes do possess a peptidoglycan cell wall. 2015, 6:7116 Nat Commun
Journal:
Nature communications
Issue Date:
2015
URI:
http://hdl.handle.net/10033/612299
DOI:
10.1038/ncomms8116
PubMed ID:
25964217
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorJeske, Olgaen
dc.contributor.authorSchüler, Margareteen
dc.contributor.authorSchumann, Peteren
dc.contributor.authorSchneider, Alexanderen
dc.contributor.authorBoedeker, Christianen
dc.contributor.authorJogler, Mareikeen
dc.contributor.authorBollschweiler, Danielen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorMayer, Christophen
dc.contributor.authorEngelhardt, Haralden
dc.contributor.authorSpring, Stefanen
dc.contributor.authorJogler, Christianen
dc.date.accessioned2016-06-09T10:40:58Zen
dc.date.available2016-06-09T10:40:58Zen
dc.date.issued2015en
dc.identifier.citationPlanctomycetes do possess a peptidoglycan cell wall. 2015, 6:7116 Nat Communen
dc.identifier.issn2041-1723en
dc.identifier.pmid25964217en
dc.identifier.doi10.1038/ncomms8116en
dc.identifier.urihttp://hdl.handle.net/10033/612299en
dc.description.abstractMost bacteria contain a peptidoglycan (PG) cell wall, which is critical for maintenance of shape and important for cell division. In contrast, Planctomycetes have been proposed to produce a proteinaceous cell wall devoid of PG. The apparent absence of PG has been used as an argument for the putative planctomycetal ancestry of all bacterial lineages. Here we show, employing multiple bioinformatic methods, that planctomycetal genomes encode proteins required for PG synthesis. Furthermore, we biochemically demonstrate the presence of the sugar and the peptide components of PG in Planctomycetes. In addition, light and electron microscopic experiments reveal planctomycetal PG sacculi that are susceptible to lysozyme treatment. Finally, cryo-electron tomography demonstrates that Planctomycetes possess a typical PG cell wall and that their cellular architecture is thus more similar to that of other Gram-negative bacteria. Our findings shed new light on the cellular architecture and cell division of the maverick Planctomycetes.en
dc.language.isoenen
dc.subject.meshAcetylglucosamineen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGene Expression Regulation, Enzymologicen
dc.subject.meshGenome, Bacterialen
dc.subject.meshMuramic Acidsen
dc.subject.meshPeptidoglycanen
dc.subject.meshPhylogenyen
dc.subject.meshPlanctomycetalesen
dc.subject.meshbeta-Lactamasesen
dc.titlePlanctomycetes do possess a peptidoglycan cell wall.en
dc.typeArticleen
dc.contributor.departmentHelmholtzzentrum für Infektionsforschung 38124 Braunschweigen
dc.identifier.journalNature communicationsen

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