2.50
Hdl Handle:
http://hdl.handle.net/10033/612301
Title:
RNA structure determination by solid-state NMR spectroscopy.
Authors:
Marchanka, Alexander; Simon, Bernd; Althoff-Ospelt, Gerhard; Carlomagno, Teresa ( 0000-0002-2437-2760 )
Abstract:
Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize—and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.
Affiliation:
Helmholtzzentrum für Infektionsforschung, 38124 Braunschweig
Citation:
RNA structure determination by solid-state NMR spectroscopy. 2015, 6:7024 Nat Commun
Journal:
Nature communications
Issue Date:
2015
URI:
http://hdl.handle.net/10033/612301
DOI:
10.1038/ncomms8024
PubMed ID:
25960310
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
publications of te research group NMR-based structural chemistry (NBSC)

Full metadata record

DC FieldValue Language
dc.contributor.authorMarchanka, Alexanderen
dc.contributor.authorSimon, Bernden
dc.contributor.authorAlthoff-Ospelt, Gerharden
dc.contributor.authorCarlomagno, Teresaen
dc.date.accessioned2016-06-09T10:47:51Zen
dc.date.available2016-06-09T10:47:51Zen
dc.date.issued2015en
dc.identifier.citationRNA structure determination by solid-state NMR spectroscopy. 2015, 6:7024 Nat Communen
dc.identifier.issn2041-1723en
dc.identifier.pmid25960310en
dc.identifier.doi10.1038/ncomms8024en
dc.identifier.urihttp://hdl.handle.net/10033/612301en
dc.description.abstractKnowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize—and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.en
dc.language.isoenen
dc.subject.meshMagnetic Resonance Spectroscopyen
dc.subject.meshModels, Molecularen
dc.subject.meshNucleic Acid Conformationen
dc.subject.meshRNAen
dc.subject.meshRibonucleoproteinsen
dc.titleRNA structure determination by solid-state NMR spectroscopy.en
dc.typeArticleen
dc.contributor.departmentHelmholtzzentrum für Infektionsforschung, 38124 Braunschweigen
dc.identifier.journalNature communicationsen

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