Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria.

2.50
Hdl Handle:
http://hdl.handle.net/10033/614065
Title:
Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria.
Authors:
Klotz, Ulrike; Schmidt, Dirk; Willinger, Birgit; Steinmann, Eike; Buer, Jan; Rath, Peter-Michael; Steinmann, Joerg
Abstract:
Echinocandin resistance in Candida glabrata is emerging and is associated with the presence of FKS mutations. In this study, we analysed the antifungal susceptibility, presence of FKS mutations and clonality of C. glabrata blood culture isolates from two hospitals in Germany and Austria. Susceptibility testing of 64 C. glabrata bloodstream isolates from two university hospitals was performed with broth microdilution method according to EUCAST. In addition, all isolates were screened for FKS mutations. Molecular fingerprinting was performed by microsatellite PCR with three separate primer pairs and semiautomated repetitive sequenced-based PCR (rep-PCR). One C. glabrata isolate from Germany (1.5%) was echinocandin resistant, with a corresponding mutation in FKS2 gene hot spot 1. The discriminatory power of microsatellite PCR was higher than that of rep-PCR (Simpson Index of 0.94 vs. 0.88); microsatellite PCR created 31 separate genotypes, whereas rep-PCR created 17. Predominant genotypes or clusters of isolates from Germany and Austria were present, with no epidemiological evidence of nosocomial transmissions. Although we found a low incidence of echinocandin resistance in C. glabrata in our settings, further surveillance projects in central Europe are warranted for monitoring future epidemiological trends. The genetic population structure of C. glabrata demonstrates overrepresented geographical clusters.
Affiliation:
Twincore Centre for Experimental and Clinical Infection Research, Hannover, Germany.
Citation:
Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria. 2016, 59 (5):312-8 Mycoses
Journal:
Mycoses
Issue Date:
May-2016
URI:
http://hdl.handle.net/10033/614065
DOI:
10.1111/myc.12472
PubMed ID:
26806376
Type:
Article
Language:
en
ISSN:
1439-0507
Appears in Collections:
publications of the research group virus transmission ([TC]VIRT)

Full metadata record

DC FieldValue Language
dc.contributor.authorKlotz, Ulrikeen
dc.contributor.authorSchmidt, Dirken
dc.contributor.authorWillinger, Birgiten
dc.contributor.authorSteinmann, Eikeen
dc.contributor.authorBuer, Janen
dc.contributor.authorRath, Peter-Michaelen
dc.contributor.authorSteinmann, Joergen
dc.date.accessioned2016-06-22T12:04:00Zen
dc.date.available2016-06-22T12:04:00Zen
dc.date.issued2016-05en
dc.identifier.citationEchinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria. 2016, 59 (5):312-8 Mycosesen
dc.identifier.issn1439-0507en
dc.identifier.pmid26806376en
dc.identifier.doi10.1111/myc.12472en
dc.identifier.urihttp://hdl.handle.net/10033/614065en
dc.description.abstractEchinocandin resistance in Candida glabrata is emerging and is associated with the presence of FKS mutations. In this study, we analysed the antifungal susceptibility, presence of FKS mutations and clonality of C. glabrata blood culture isolates from two hospitals in Germany and Austria. Susceptibility testing of 64 C. glabrata bloodstream isolates from two university hospitals was performed with broth microdilution method according to EUCAST. In addition, all isolates were screened for FKS mutations. Molecular fingerprinting was performed by microsatellite PCR with three separate primer pairs and semiautomated repetitive sequenced-based PCR (rep-PCR). One C. glabrata isolate from Germany (1.5%) was echinocandin resistant, with a corresponding mutation in FKS2 gene hot spot 1. The discriminatory power of microsatellite PCR was higher than that of rep-PCR (Simpson Index of 0.94 vs. 0.88); microsatellite PCR created 31 separate genotypes, whereas rep-PCR created 17. Predominant genotypes or clusters of isolates from Germany and Austria were present, with no epidemiological evidence of nosocomial transmissions. Although we found a low incidence of echinocandin resistance in C. glabrata in our settings, further surveillance projects in central Europe are warranted for monitoring future epidemiological trends. The genetic population structure of C. glabrata demonstrates overrepresented geographical clusters.en
dc.language.isoenen
dc.titleEchinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria.en
dc.typeArticleen
dc.contributor.departmentTwincore Centre for Experimental and Clinical Infection Research, Hannover, Germany.en
dc.identifier.journalMycosesen

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