What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?

2.50
Hdl Handle:
http://hdl.handle.net/10033/615464
Title:
What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?
Authors:
Bárcia, R N; Santos, J M; Filipe, M; Teixeira, M; Martins, J P; Almeida, J; Água-Doce, A; Almeida, S C P; Varela, A; Pohl, S; Dittmar, K E J; Calado, S; Simões, S I; Gaspar, M M; Cruz, M E M; Lindenmaier, W; Graça, L; Cruz, H; Cruz, P E
Abstract:
MSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of lymphocyte proliferation and induction of regulatory T cells. Results showed that UCX were less immunogenic and showed higher immunosuppression activity than BM-MSCs. Further, UCX did not need prior activation or priming to exert their immunomodulatory effects. This was further corroborated in vivo in a model of acute inflammation. To elucidate the potency differences observed between UCX and BM-MSCs, gene expression related to immune modulation was analysed in both cell types. Several gene expression profile differences were found between UCX and BM-MSCs, namely decreased expression of HLA-DRA, HO-1, IGFBP1, 4 and 6, ILR1, IL6R and PTGES and increased expression of CD200, CD273, CD274, IL1B, IL-8, LIF and TGFB2. The latter were confirmed at the protein expression level. Overall, these results show that UCX seem to be naturally more potent immunosuppressors and less immunogenic than BM-MSCs. We propose that these differences may be due to increased levels of immunomodulatory surface proteins such as CD200, CD273, CD274 and cytokines such as IL1β, IL-8, LIF and TGFβ2.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? 2015, 2015:583984 Stem Cells Int
Journal:
Stem cells international
Issue Date:
2015
URI:
http://hdl.handle.net/10033/615464
DOI:
10.1155/2015/583984
PubMed ID:
26064137
Type:
Article
Language:
en
ISSN:
1687-966X
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorBárcia, R Nen
dc.contributor.authorSantos, J Men
dc.contributor.authorFilipe, Men
dc.contributor.authorTeixeira, Men
dc.contributor.authorMartins, J Pen
dc.contributor.authorAlmeida, Jen
dc.contributor.authorÁgua-Doce, Aen
dc.contributor.authorAlmeida, S C Pen
dc.contributor.authorVarela, Aen
dc.contributor.authorPohl, Sen
dc.contributor.authorDittmar, K E Jen
dc.contributor.authorCalado, Sen
dc.contributor.authorSimões, S Ien
dc.contributor.authorGaspar, M Men
dc.contributor.authorCruz, M E Men
dc.contributor.authorLindenmaier, Wen
dc.contributor.authorGraça, Len
dc.contributor.authorCruz, Hen
dc.contributor.authorCruz, P Een
dc.date.accessioned2016-07-04T13:23:51Z-
dc.date.available2016-07-04T13:23:51Z-
dc.date.issued2015-
dc.identifier.citationWhat Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? 2015, 2015:583984 Stem Cells Inten
dc.identifier.issn1687-966X-
dc.identifier.pmid26064137-
dc.identifier.doi10.1155/2015/583984-
dc.identifier.urihttp://hdl.handle.net/10033/615464-
dc.description.abstractMSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of lymphocyte proliferation and induction of regulatory T cells. Results showed that UCX were less immunogenic and showed higher immunosuppression activity than BM-MSCs. Further, UCX did not need prior activation or priming to exert their immunomodulatory effects. This was further corroborated in vivo in a model of acute inflammation. To elucidate the potency differences observed between UCX and BM-MSCs, gene expression related to immune modulation was analysed in both cell types. Several gene expression profile differences were found between UCX and BM-MSCs, namely decreased expression of HLA-DRA, HO-1, IGFBP1, 4 and 6, ILR1, IL6R and PTGES and increased expression of CD200, CD273, CD274, IL1B, IL-8, LIF and TGFB2. The latter were confirmed at the protein expression level. Overall, these results show that UCX seem to be naturally more potent immunosuppressors and less immunogenic than BM-MSCs. We propose that these differences may be due to increased levels of immunomodulatory surface proteins such as CD200, CD273, CD274 and cytokines such as IL1β, IL-8, LIF and TGFβ2.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleWhat Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalStem cells internationalen

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