2.50
Hdl Handle:
http://hdl.handle.net/10033/615465
Title:
Biodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2.
Authors:
Poth, Nils; Seiffart, Virginia; Gross, Gerhard; Menzel, Henning; Dempwolf, Wibke
Abstract:
A simple method for the functionalization of a common implant material (Ti6Al4V) with biodegradable, drug loaded chitosan-tripolyphosphate (CS-TPP) nanoparticles is developed in order to enhance the osseointegration of endoprostheses after revision operations. The chitosan used has a tailored degree of acetylation which allows for a fast biodegradation by lysozyme. The degradability of chitosan is proven via viscometry. Characteristics and degradation of nanoparticles formed with TPP are analyzed using dynamic light scattering. The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days. Drug loading and release is investigated for the nanoparticles with bone morphogenetic protein 2 (BMP-2), using ELISA and the BRE luciferase test for quantification and bioactivity evaluation. Furthermore, nanoparticle coatings on titanium substrates are created via spray-coating and analyzed by ellipsometry, scanning electron microscopy and X-ray photoelectron spectroscopy. Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work. Additionally, an in vivo study in mice indicates the dose dependent induction of ectopic bone growth through CS-TPP-BMP-2 nanoparticles. These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Biodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2. 2015, 5 (1):3-19 Biomolecules
Journal:
Biomolecules
Issue Date:
2015
URI:
http://hdl.handle.net/10033/615465
DOI:
10.3390/biom5010003
PubMed ID:
25581889
Type:
Article
Language:
en
ISSN:
2218-273X
Appears in Collections:
publications of the research group immunoregulation (IREG)

Full metadata record

DC FieldValue Language
dc.contributor.authorPoth, Nilsen
dc.contributor.authorSeiffart, Virginiaen
dc.contributor.authorGross, Gerharden
dc.contributor.authorMenzel, Henningen
dc.contributor.authorDempwolf, Wibkeen
dc.date.accessioned2016-07-04T14:04:57Z-
dc.date.available2016-07-04T14:04:57Z-
dc.date.issued2015-
dc.identifier.citationBiodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2. 2015, 5 (1):3-19 Biomoleculesen
dc.identifier.issn2218-273X-
dc.identifier.pmid25581889-
dc.identifier.doi10.3390/biom5010003-
dc.identifier.urihttp://hdl.handle.net/10033/615465-
dc.description.abstractA simple method for the functionalization of a common implant material (Ti6Al4V) with biodegradable, drug loaded chitosan-tripolyphosphate (CS-TPP) nanoparticles is developed in order to enhance the osseointegration of endoprostheses after revision operations. The chitosan used has a tailored degree of acetylation which allows for a fast biodegradation by lysozyme. The degradability of chitosan is proven via viscometry. Characteristics and degradation of nanoparticles formed with TPP are analyzed using dynamic light scattering. The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days. Drug loading and release is investigated for the nanoparticles with bone morphogenetic protein 2 (BMP-2), using ELISA and the BRE luciferase test for quantification and bioactivity evaluation. Furthermore, nanoparticle coatings on titanium substrates are created via spray-coating and analyzed by ellipsometry, scanning electron microscopy and X-ray photoelectron spectroscopy. Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work. Additionally, an in vivo study in mice indicates the dose dependent induction of ectopic bone growth through CS-TPP-BMP-2 nanoparticles. These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAcetylationen
dc.subject.meshAnimalsen
dc.subject.meshBone Morphogenetic Protein 2en
dc.subject.meshCell Lineen
dc.subject.meshChitosanen
dc.subject.meshCoated Materials, Biocompatibleen
dc.subject.meshDrug Carriersen
dc.subject.meshDrug Liberationen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshNanoparticlesen
dc.subject.meshOsseointegrationen
dc.subject.meshPolyphosphatesen
dc.subject.meshProstheses and Implantsen
dc.subject.meshSurface Propertiesen
dc.subject.meshTitaniumen
dc.titleBiodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBiomoleculesen
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