2.50
Hdl Handle:
http://hdl.handle.net/10033/615660
Title:
Alterations of the Murine Gut Microbiome with Age and Allergic Airway Disease.
Authors:
Vital, Marius ( 0000-0003-4185-3475 ) ; Harkema, Jack R; Rizzo, Mike; Tiedje, James; Brandenberger, Christina
Abstract:
The gut microbiota plays an important role in the development of asthma. With advanced age the microbiome and the immune system are changing and, currently, little is known about how these two factors contribute to the development of allergic asthma in the elderly. In this study we investigated the associations between the intestinal microbiome and allergic airway disease in young and old mice that were sensitized and challenged with house dust mite (HDM). After challenge, the animals were sacrificed, blood serum was collected for cytokine analysis, and the lungs were processed for histopathology. Fecal pellets were excised from the colon and subjected to 16S rRNA analysis. The microbial community structure changed with age and allergy development, where alterations in fecal communities from young to old mice resembled those after HDM challenge. Allergic mice had induced serum levels of IL-17A and old mice developed a greater allergic airway response compared to young mice. This study demonstrates that the intestinal bacterial community structure differs with age, possibly contributing to the exaggerated pulmonary inflammatory response in old mice. Furthermore, our results show that the composition of the gut microbiota changes with pulmonary allergy, indicating bidirectional gut-lung communications.
Affiliation:
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
Citation:
Alterations of the Murine Gut Microbiome with Age and Allergic Airway Disease. 2015, 2015:892568 J Immunol Res
Journal:
Journal of immunology research
Issue Date:
2015
URI:
http://hdl.handle.net/10033/615660
DOI:
10.1155/2015/892568
PubMed ID:
26090504
Type:
Article
Language:
en
ISSN:
2314-7156
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorVital, Mariusen
dc.contributor.authorHarkema, Jack Ren
dc.contributor.authorRizzo, Mikeen
dc.contributor.authorTiedje, Jamesen
dc.contributor.authorBrandenberger, Christinaen
dc.date.accessioned2016-07-06T14:44:58Z-
dc.date.available2016-07-06T14:44:58Z-
dc.date.issued2015-
dc.identifier.citationAlterations of the Murine Gut Microbiome with Age and Allergic Airway Disease. 2015, 2015:892568 J Immunol Resen
dc.identifier.issn2314-7156-
dc.identifier.pmid26090504-
dc.identifier.doi10.1155/2015/892568-
dc.identifier.urihttp://hdl.handle.net/10033/615660-
dc.description.abstractThe gut microbiota plays an important role in the development of asthma. With advanced age the microbiome and the immune system are changing and, currently, little is known about how these two factors contribute to the development of allergic asthma in the elderly. In this study we investigated the associations between the intestinal microbiome and allergic airway disease in young and old mice that were sensitized and challenged with house dust mite (HDM). After challenge, the animals were sacrificed, blood serum was collected for cytokine analysis, and the lungs were processed for histopathology. Fecal pellets were excised from the colon and subjected to 16S rRNA analysis. The microbial community structure changed with age and allergy development, where alterations in fecal communities from young to old mice resembled those after HDM challenge. Allergic mice had induced serum levels of IL-17A and old mice developed a greater allergic airway response compared to young mice. This study demonstrates that the intestinal bacterial community structure differs with age, possibly contributing to the exaggerated pulmonary inflammatory response in old mice. Furthermore, our results show that the composition of the gut microbiota changes with pulmonary allergy, indicating bidirectional gut-lung communications.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAge Factorsen
dc.subject.meshAnimalsen
dc.subject.meshAsthmaen
dc.subject.meshDisease Models, Animalen
dc.subject.meshGastrointestinal Microbiomeen
dc.subject.meshHypersensitivityen
dc.subject.meshInterleukin-17en
dc.subject.meshIntestinesen
dc.subject.meshLungen
dc.subject.meshMaleen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshPneumoniaen
dc.subject.meshPyroglyphidaeen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.titleAlterations of the Murine Gut Microbiome with Age and Allergic Airway Disease.en
dc.typeArticleen
dc.contributor.departmentCenter for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.en
dc.identifier.journalJournal of immunology researchen

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