Deep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation.
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Authors
Bruchmann, SebastianMuthukumarasamy, Uthayakumar
Pohl, Sarah
Preusse, Matthias
Bielecka, Agata
Nicolai, Tanja
Hamann, Isabell
Hillert, Roger
Kola, Axel
Gastmeier, Petra
Eckweiler, Denitsa
Häussler, Susanne
Issue Date
2015-11
Metadata
Show full item recordAbstract
Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait.Citation
Deep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation. 2015, 17 (11):4690-710 Environ. Microbiol.Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.Journal
Environmental microbiologyPubMed ID
26261087Type
ArticleLanguage
enISSN
1462-2920ae974a485f413a2113503eed53cd6c53
10.1111/1462-2920.13016
Scopus Count
The following license files are associated with this item:
- Creative Commons
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