A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.

2.50
Hdl Handle:
http://hdl.handle.net/10033/618520
Title:
A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.
Authors:
Koeppen, Katja; Hampton, Thomas H; Jarek, Michael; Scharfe, Maren; Gerber, Scott A; Mielcarz, Daniel W; Demers, Elora G; Dolben, Emily L; Hammond, John H; Hogan, Deborah A; Stanton, Bruce A
Abstract:
Bacterial outer membrane vesicle (OMV)-mediated delivery of proteins to host cells is an important mechanism of host-pathogen communication. Emerging evidence suggests that OMVs contain differentially packaged short RNAs (sRNAs) with the potential to target host mRNA function and/or stability. In this study, we used RNA-Seq to characterize differentially packaged sRNAs in Pseudomonas aeruginosa OMVs, and to show transfer of OMV sRNAs to human airway cells. We selected one sRNA for further study based on its stable secondary structure and predicted mRNA targets. Our candidate sRNA (sRNA52320), a fragment of a P. aeruginosa methionine tRNA, was abundant in OMVs and reduced LPS-induced as well as OMV-induced IL-8 secretion by cultured primary human airway epithelial cells. We also showed that sRNA52320 attenuated OMV-induced KC cytokine secretion and neutrophil infiltration in mouse lung. Collectively, these findings are consistent with the hypothesis that sRNA52320 in OMVs is a novel mechanism of host-pathogen interaction whereby P. aeruginosa reduces the host immune response.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles. 2016, 12 (6):e1005672 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
Jun-2016
URI:
http://hdl.handle.net/10033/618520
DOI:
10.1371/journal.ppat.1005672
PubMed ID:
27295279
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorKoeppen, Katjaen
dc.contributor.authorHampton, Thomas Hen
dc.contributor.authorJarek, Michaelen
dc.contributor.authorScharfe, Marenen
dc.contributor.authorGerber, Scott Aen
dc.contributor.authorMielcarz, Daniel Wen
dc.contributor.authorDemers, Elora Gen
dc.contributor.authorDolben, Emily Len
dc.contributor.authorHammond, John Hen
dc.contributor.authorHogan, Deborah Aen
dc.contributor.authorStanton, Bruce Aen
dc.date.accessioned2016-08-18T09:35:46Z-
dc.date.available2016-08-18T09:35:46Z-
dc.date.issued2016-06-
dc.identifier.citationA Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles. 2016, 12 (6):e1005672 PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid27295279-
dc.identifier.doi10.1371/journal.ppat.1005672-
dc.identifier.urihttp://hdl.handle.net/10033/618520-
dc.description.abstractBacterial outer membrane vesicle (OMV)-mediated delivery of proteins to host cells is an important mechanism of host-pathogen communication. Emerging evidence suggests that OMVs contain differentially packaged short RNAs (sRNAs) with the potential to target host mRNA function and/or stability. In this study, we used RNA-Seq to characterize differentially packaged sRNAs in Pseudomonas aeruginosa OMVs, and to show transfer of OMV sRNAs to human airway cells. We selected one sRNA for further study based on its stable secondary structure and predicted mRNA targets. Our candidate sRNA (sRNA52320), a fragment of a P. aeruginosa methionine tRNA, was abundant in OMVs and reduced LPS-induced as well as OMV-induced IL-8 secretion by cultured primary human airway epithelial cells. We also showed that sRNA52320 attenuated OMV-induced KC cytokine secretion and neutrophil infiltration in mouse lung. Collectively, these findings are consistent with the hypothesis that sRNA52320 in OMVs is a novel mechanism of host-pathogen interaction whereby P. aeruginosa reduces the host immune response.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleA Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen

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