Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers.
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Authors
Planz, ViktoriaSeif, Salem
Atchison, Jennifer S
Vukosavljevic, Branko
Sparenberg, Lisa
Kroner, Elmar
Windbergs, Maike
Issue Date
2016-07-11
Metadata
Show full item recordAbstract
The human skin comprises a complex multi-scale layered structure with hierarchical organization of different cells within the extracellular matrix (ECM). This supportive fiber-reinforced structure provides a dynamically changing microenvironment with specific topographical, mechanical and biochemical cell recognition sites to facilitate cell attachment and proliferation. Current advances in developing artificial matrices for cultivation of human cells concentrate on surface functionalizing of biocompatible materials with different biomolecules like growth factors to enhance cell attachment. However, an often neglected aspect for efficient modulation of cell-matrix interactions is posed by the mechanical characteristics of such artificial matrices. To address this issue, we fabricated biocompatible hybrid fibers simulating the complex biomechanical characteristics of native ECM in human skin. Subsequently, we analyzed interactions of such fibers with human skin cells focusing on the identification of key fiber characteristics for optimized cell-matrix interactions. We successfully identified the mediating effect of bio-adaptive elasto-plastic stiffness paired with hydrophilic surface properties as key factors for cell attachment and proliferation, thus elucidating the synergistic role of these parameters to induce cellular responses. Co-cultivation of fibroblasts and keratinocytes on such fiber mats representing the specific cells in dermis and epidermis resulted in a hierarchical organization of dermal and epidermal tissue layers. In addition, terminal differentiation of keratinocytes at the air interface was observed. These findings provide valuable new insights into cell behaviour in three-dimensional structures and cell-material interactions which can be used for rational development of bio-inspired functional materials for advanced biomedical applications.Citation
Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers. 2016, 8 (7):775-84 Integr Biol (Camb)Affiliation
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.PubMed ID
27241237Type
ArticleLanguage
enISSN
1757-9708ae974a485f413a2113503eed53cd6c53
10.1039/c6ib00080k
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
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