Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers.

2.50
Hdl Handle:
http://hdl.handle.net/10033/618602
Title:
Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers.
Authors:
Planz, Viktoria; Seif, Salem; Atchison, Jennifer S; Vukosavljevic, Branko; Sparenberg, Lisa; Kroner, Elmar; Windbergs, Maike
Abstract:
The human skin comprises a complex multi-scale layered structure with hierarchical organization of different cells within the extracellular matrix (ECM). This supportive fiber-reinforced structure provides a dynamically changing microenvironment with specific topographical, mechanical and biochemical cell recognition sites to facilitate cell attachment and proliferation. Current advances in developing artificial matrices for cultivation of human cells concentrate on surface functionalizing of biocompatible materials with different biomolecules like growth factors to enhance cell attachment. However, an often neglected aspect for efficient modulation of cell-matrix interactions is posed by the mechanical characteristics of such artificial matrices. To address this issue, we fabricated biocompatible hybrid fibers simulating the complex biomechanical characteristics of native ECM in human skin. Subsequently, we analyzed interactions of such fibers with human skin cells focusing on the identification of key fiber characteristics for optimized cell-matrix interactions. We successfully identified the mediating effect of bio-adaptive elasto-plastic stiffness paired with hydrophilic surface properties as key factors for cell attachment and proliferation, thus elucidating the synergistic role of these parameters to induce cellular responses. Co-cultivation of fibroblasts and keratinocytes on such fiber mats representing the specific cells in dermis and epidermis resulted in a hierarchical organization of dermal and epidermal tissue layers. In addition, terminal differentiation of keratinocytes at the air interface was observed. These findings provide valuable new insights into cell behaviour in three-dimensional structures and cell-material interactions which can be used for rational development of bio-inspired functional materials for advanced biomedical applications.
Affiliation:
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers. 2016, 8 (7):775-84 Integr Biol (Camb)
Journal:
Integrative biology : quantitative biosciences from nano to macro
Issue Date:
11-Jul-2016
URI:
http://hdl.handle.net/10033/618602
DOI:
10.1039/c6ib00080k
PubMed ID:
27241237
Type:
Article
Language:
en
ISSN:
1757-9708
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorPlanz, Viktoriaen
dc.contributor.authorSeif, Salemen
dc.contributor.authorAtchison, Jennifer Sen
dc.contributor.authorVukosavljevic, Brankoen
dc.contributor.authorSparenberg, Lisaen
dc.contributor.authorKroner, Elmaren
dc.contributor.authorWindbergs, Maikeen
dc.date.accessioned2016-08-19T13:52:06Z-
dc.date.available2016-08-19T13:52:06Z-
dc.date.issued2016-07-11-
dc.identifier.citationThree-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers. 2016, 8 (7):775-84 Integr Biol (Camb)en
dc.identifier.issn1757-9708-
dc.identifier.pmid27241237-
dc.identifier.doi10.1039/c6ib00080k-
dc.identifier.urihttp://hdl.handle.net/10033/618602-
dc.description.abstractThe human skin comprises a complex multi-scale layered structure with hierarchical organization of different cells within the extracellular matrix (ECM). This supportive fiber-reinforced structure provides a dynamically changing microenvironment with specific topographical, mechanical and biochemical cell recognition sites to facilitate cell attachment and proliferation. Current advances in developing artificial matrices for cultivation of human cells concentrate on surface functionalizing of biocompatible materials with different biomolecules like growth factors to enhance cell attachment. However, an often neglected aspect for efficient modulation of cell-matrix interactions is posed by the mechanical characteristics of such artificial matrices. To address this issue, we fabricated biocompatible hybrid fibers simulating the complex biomechanical characteristics of native ECM in human skin. Subsequently, we analyzed interactions of such fibers with human skin cells focusing on the identification of key fiber characteristics for optimized cell-matrix interactions. We successfully identified the mediating effect of bio-adaptive elasto-plastic stiffness paired with hydrophilic surface properties as key factors for cell attachment and proliferation, thus elucidating the synergistic role of these parameters to induce cellular responses. Co-cultivation of fibroblasts and keratinocytes on such fiber mats representing the specific cells in dermis and epidermis resulted in a hierarchical organization of dermal and epidermal tissue layers. In addition, terminal differentiation of keratinocytes at the air interface was observed. These findings provide valuable new insights into cell behaviour in three-dimensional structures and cell-material interactions which can be used for rational development of bio-inspired functional materials for advanced biomedical applications.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleThree-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalIntegrative biology : quantitative biosciences from nano to macroen

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