In Vivo Conditions Enable IFNAR-Independent Type I Interferon Production by Peritoneal CD11b+ Cells upon Thogoto Virus Infection.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620545
Title:
In Vivo Conditions Enable IFNAR-Independent Type I Interferon Production by Peritoneal CD11b+ Cells upon Thogoto Virus Infection.
Authors:
Kochs, Georg; Anzaghe, Martina; Kronhart, Stefanie; Wagner, Valentina; Gogesch, Patricia; Scheu, Stefanie; Lienenklaus, Stefan ( 0000-0001-6141-9102 ) ; Waibler, Zoe
Abstract:
Type I interferons (IFNs) crucially contribute to host survival upon viral infections. Robust expression of type I IFNs (IFN-α/β) and induction of an antiviral state critically depend on amplification of the IFN signal via the type I IFN receptor (IFNAR). A small amount of type I IFN produced early upon virus infection binds the IFNAR and activates a self-enhancing positive feedback loop, resulting in induction of large, protective amounts of IFN-α. Unexpectedly, we found robust, systemic IFN-α expression upon infection of IFNAR knockout mice with the orthomyxovirus Thogoto virus (THOV). The IFNAR-independent IFN-α production required in vivo conditions and was not achieved during in vitro infection. Using replication-incompetent THOV-derived virus-like particles, we demonstrate that IFNAR-independent type I IFN induction depends on viral polymerase activity but is largely independent of viral replication. To discover the cell type responsible for this effect, we used type I IFN reporter mice and identified CD11b(+) F4/80(+) myeloid cells within the peritoneal cavity of infected animals as the main source of IFNAR-independent type I IFN, corresponding to the particular tropism of THOV for this cell type.
Affiliation:
Medizinische Hochschule Hannover: Hannover, Niedersachsen, Germany.
Citation:
In Vivo Conditions Enable IFNAR-Independent Type I Interferon Production by Peritoneal CD11b+ Cells upon Thogoto Virus Infection. 2016, 90 (20):9330-7 J. Virol.
Journal:
Journal of virology
Issue Date:
15-Oct-2016
URI:
http://hdl.handle.net/10033/620545
DOI:
10.1128/JVI.00744-16
PubMed ID:
27512061
Type:
Article
Language:
en
ISSN:
1098-5514
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorKochs, Georgen
dc.contributor.authorAnzaghe, Martinaen
dc.contributor.authorKronhart, Stefanieen
dc.contributor.authorWagner, Valentinaen
dc.contributor.authorGogesch, Patriciaen
dc.contributor.authorScheu, Stefanieen
dc.contributor.authorLienenklaus, Stefanen
dc.contributor.authorWaibler, Zoeen
dc.date.accessioned2016-10-10T14:06:18Z-
dc.date.available2016-10-10T14:06:18Z-
dc.date.issued2016-10-15-
dc.identifier.citationIn Vivo Conditions Enable IFNAR-Independent Type I Interferon Production by Peritoneal CD11b+ Cells upon Thogoto Virus Infection. 2016, 90 (20):9330-7 J. Virol.en
dc.identifier.issn1098-5514-
dc.identifier.pmid27512061-
dc.identifier.doi10.1128/JVI.00744-16-
dc.identifier.urihttp://hdl.handle.net/10033/620545-
dc.description.abstractType I interferons (IFNs) crucially contribute to host survival upon viral infections. Robust expression of type I IFNs (IFN-α/β) and induction of an antiviral state critically depend on amplification of the IFN signal via the type I IFN receptor (IFNAR). A small amount of type I IFN produced early upon virus infection binds the IFNAR and activates a self-enhancing positive feedback loop, resulting in induction of large, protective amounts of IFN-α. Unexpectedly, we found robust, systemic IFN-α expression upon infection of IFNAR knockout mice with the orthomyxovirus Thogoto virus (THOV). The IFNAR-independent IFN-α production required in vivo conditions and was not achieved during in vitro infection. Using replication-incompetent THOV-derived virus-like particles, we demonstrate that IFNAR-independent type I IFN induction depends on viral polymerase activity but is largely independent of viral replication. To discover the cell type responsible for this effect, we used type I IFN reporter mice and identified CD11b(+) F4/80(+) myeloid cells within the peritoneal cavity of infected animals as the main source of IFNAR-independent type I IFN, corresponding to the particular tropism of THOV for this cell type.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn Vivo Conditions Enable IFNAR-Independent Type I Interferon Production by Peritoneal CD11b+ Cells upon Thogoto Virus Infection.en
dc.typeArticleen
dc.contributor.departmentMedizinische Hochschule Hannover: Hannover, Niedersachsen, Germany.en
dc.identifier.journalJournal of virologyen

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