Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells.

5.00
Hdl Handle:
http://hdl.handle.net/10033/620631
Title:
Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells.
Authors:
Zakrzewicz, Dariusz; Bergmann, Simone; Didiasova, Miroslava; Giaimo, Benedetto Daniele; Borggrefe, Tilman; Mieth, Maren; Hocke, Andreas C; Lochnit, Guenter; Schaefer, Liliana; Hammerschmidt, Sven; Preissner, Klaus T; Wygrecka, Malgorzata
Abstract:
Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. The infection process involves bacterial cell surface receptors, which interact with host extracellular matrix components to facilitate colonization and dissemination of bacteria. Here, we investigated the role of host-derived extracellular RNA (eRNA) in the process of pneumococcal alveolar epithelial cell infection. Our study demonstrates that eRNA dose-dependently increased S. pneumoniae invasion of alveolar epithelial cells. Extracellular enolase (Eno), a plasminogen (Plg) receptor, was identified as a novel eRNA-binding protein on S. pneumoniae surface, and six Eno eRNA-binding sites including a C-terminal 15 amino acid motif containing lysine residue 434 were characterized. Although the substitution of lysine 434 for glycine (K434G) markedly diminished the binding of eRNA to Eno, the adherence to and internalization into alveolar epithelial cells of S. pneumoniae strain carrying the C-terminal lysine deletion and the mutation of internal Plg-binding motif were only marginally impaired. Accordingly, using a mass spectrometric approach, we identified seven novel eRNA-binding proteins in pneumococcal cell wall. Given the high number of eRNA-interacting proteins on pneumococci, treatment with RNase1 completely inhibited eRNA-mediated pneumococcal alveolar epithelial cell infection. Our data support further efforts to employ RNAse1 as an antimicrobial agent to combat pneumococcal infectious diseases.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells. 2016, 6:37758 Sci Rep
Journal:
Scientific reports
Issue Date:
28-Nov-2016
URI:
http://hdl.handle.net/10033/620631
DOI:
10.1038/srep37758
PubMed ID:
27892961
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorZakrzewicz, Dariuszen
dc.contributor.authorBergmann, Simoneen
dc.contributor.authorDidiasova, Miroslavaen
dc.contributor.authorGiaimo, Benedetto Danieleen
dc.contributor.authorBorggrefe, Tilmanen
dc.contributor.authorMieth, Marenen
dc.contributor.authorHocke, Andreas Cen
dc.contributor.authorLochnit, Guenteren
dc.contributor.authorSchaefer, Lilianaen
dc.contributor.authorHammerschmidt, Svenen
dc.contributor.authorPreissner, Klaus Ten
dc.contributor.authorWygrecka, Malgorzataen
dc.date.accessioned2016-12-06T10:34:31Z-
dc.date.available2016-12-06T10:34:31Z-
dc.date.issued2016-11-28-
dc.identifier.citationHost-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells. 2016, 6:37758 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid27892961-
dc.identifier.doi10.1038/srep37758-
dc.identifier.urihttp://hdl.handle.net/10033/620631-
dc.description.abstractStreptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. The infection process involves bacterial cell surface receptors, which interact with host extracellular matrix components to facilitate colonization and dissemination of bacteria. Here, we investigated the role of host-derived extracellular RNA (eRNA) in the process of pneumococcal alveolar epithelial cell infection. Our study demonstrates that eRNA dose-dependently increased S. pneumoniae invasion of alveolar epithelial cells. Extracellular enolase (Eno), a plasminogen (Plg) receptor, was identified as a novel eRNA-binding protein on S. pneumoniae surface, and six Eno eRNA-binding sites including a C-terminal 15 amino acid motif containing lysine residue 434 were characterized. Although the substitution of lysine 434 for glycine (K434G) markedly diminished the binding of eRNA to Eno, the adherence to and internalization into alveolar epithelial cells of S. pneumoniae strain carrying the C-terminal lysine deletion and the mutation of internal Plg-binding motif were only marginally impaired. Accordingly, using a mass spectrometric approach, we identified seven novel eRNA-binding proteins in pneumococcal cell wall. Given the high number of eRNA-interacting proteins on pneumococci, treatment with RNase1 completely inhibited eRNA-mediated pneumococcal alveolar epithelial cell infection. Our data support further efforts to employ RNAse1 as an antimicrobial agent to combat pneumococcal infectious diseases.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleHost-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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