2.50
Hdl Handle:
http://hdl.handle.net/10033/620669
Title:
The PARA-suite: PAR-CLIP specific sequence read simulation and processing.
Authors:
Kloetgen, Andreas; Borkhardt, Arndt; Hoell, Jessica I; McHardy, Alice C
Abstract:
Next-generation sequencing technologies have profoundly impacted biology over recent years. Experimental protocols, such as photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP), which identifies protein-RNA interactions on a genome-wide scale, commonly employ deep sequencing. With PAR-CLIP, the incorporation of photoactivatable nucleosides into nascent transcripts leads to high rates of specific nucleotide conversions during reverse transcription. So far, the specific properties of PAR-CLIP-derived sequencing reads have not been assessed in depth.
Affiliation:
BRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56, 38106 Braunschweig, Germany.
Citation:
The PARA-suite: PAR-CLIP specific sequence read simulation and processing. 2016, 4:e2619 PeerJ
Journal:
PeerJ
Issue Date:
2016
URI:
http://hdl.handle.net/10033/620669
DOI:
10.7717/peerj.2619
PubMed ID:
27812418
Type:
Article
Language:
en
Appears in Collections:
publications of the research group bioinformatics in infection research ([BRICS] BIFO)

Full metadata record

DC FieldValue Language
dc.contributor.authorKloetgen, Andreasen
dc.contributor.authorBorkhardt, Arndten
dc.contributor.authorHoell, Jessica Ien
dc.contributor.authorMcHardy, Alice Cen
dc.date.accessioned2016-12-15T15:06:07Z-
dc.date.available2016-12-15T15:06:07Z-
dc.date.issued2016-
dc.identifier.citationThe PARA-suite: PAR-CLIP specific sequence read simulation and processing. 2016, 4:e2619 PeerJen
dc.identifier.pmid27812418-
dc.identifier.doi10.7717/peerj.2619-
dc.identifier.urihttp://hdl.handle.net/10033/620669-
dc.description.abstractNext-generation sequencing technologies have profoundly impacted biology over recent years. Experimental protocols, such as photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP), which identifies protein-RNA interactions on a genome-wide scale, commonly employ deep sequencing. With PAR-CLIP, the incorporation of photoactivatable nucleosides into nascent transcripts leads to high rates of specific nucleotide conversions during reverse transcription. So far, the specific properties of PAR-CLIP-derived sequencing reads have not been assessed in depth.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleThe PARA-suite: PAR-CLIP specific sequence read simulation and processing.en
dc.typeArticleen
dc.contributor.departmentBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56, 38106 Braunschweig, Germany.en
dc.identifier.journalPeerJen

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