Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels

2.50
Hdl Handle:
http://hdl.handle.net/10033/620762
Title:
Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels
Authors:
Schniedermann, Judith; Rennecke, Moritz; Buttler, Kerstin; Richter, Georg; Städtler, Anna-Maria; Norgall, Susanne; Badar, Muhammad; Barleon, Bernhard; May, Tobias; Wilting, Jörg; Weich, Herbert A
Abstract:
Abstract Background Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated. Results In an attempt to isolate differentiated mature endothelial cells from mouse lung we found that the lung contains EPCs with a high vasculogenic capacity and capability of de novo vasculogenesis for blood and lymph vessels. Mouse lung microvascular endothelial cells (MLMVECs) were isolated by selection of CD31+ cells. Whereas the majority of the CD31+ cells did not divide, some scattered cells started to proliferate giving rise to large colonies (> 3000 cells/colony). These highly dividing cells possess the capacity to integrate into various types of vessels including blood and lymph vessels unveiling the existence of local microvascular endothelial progenitor cells (LMEPCs) in adult mouse lung. EPCs could be amplified > passage 30 and still expressed panendothelial markers as well as the progenitor cell antigens, but not antigens for immune cells and hematopoietic stem cells. A high percentage of these cells are also positive for Lyve1, Prox1, podoplanin and VEGFR-3 indicating that a considerabe fraction of the cells are committed to develop lymphatic endothelium. Clonogenic highly proliferating cells from limiting dilution assays were also bipotent. Combined in vitro and in vivo spheroid and matrigel assays revealed that these EPCs exhibit vasculogenic capacity by forming functional blood and lymph vessels. Conclusion The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell.
Citation:
BMC Cell Biology. 2010 Jul 01;11(1):50
Issue Date:
1-Jul-2010
URI:
http://dx.doi.org/10.1186/1471-2121-11-50; http://hdl.handle.net/10033/620762
Type:
Journal Article
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchniedermann, Judithen
dc.contributor.authorRennecke, Moritzen
dc.contributor.authorButtler, Kerstinen
dc.contributor.authorRichter, Georgen
dc.contributor.authorStädtler, Anna-Mariaen
dc.contributor.authorNorgall, Susanneen
dc.contributor.authorBadar, Muhammaden
dc.contributor.authorBarleon, Bernharden
dc.contributor.authorMay, Tobiasen
dc.contributor.authorWilting, Jörgen
dc.contributor.authorWeich, Herbert Aen
dc.date.accessioned2017-01-27T09:21:04Z-
dc.date.available2017-01-27T09:21:04Z-
dc.date.issued2010-07-01en
dc.identifier.citationBMC Cell Biology. 2010 Jul 01;11(1):50en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2121-11-50en
dc.identifier.urihttp://hdl.handle.net/10033/620762-
dc.description.abstractAbstract Background Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated. Results In an attempt to isolate differentiated mature endothelial cells from mouse lung we found that the lung contains EPCs with a high vasculogenic capacity and capability of de novo vasculogenesis for blood and lymph vessels. Mouse lung microvascular endothelial cells (MLMVECs) were isolated by selection of CD31+ cells. Whereas the majority of the CD31+ cells did not divide, some scattered cells started to proliferate giving rise to large colonies (> 3000 cells/colony). These highly dividing cells possess the capacity to integrate into various types of vessels including blood and lymph vessels unveiling the existence of local microvascular endothelial progenitor cells (LMEPCs) in adult mouse lung. EPCs could be amplified > passage 30 and still expressed panendothelial markers as well as the progenitor cell antigens, but not antigens for immune cells and hematopoietic stem cells. A high percentage of these cells are also positive for Lyve1, Prox1, podoplanin and VEGFR-3 indicating that a considerabe fraction of the cells are committed to develop lymphatic endothelium. Clonogenic highly proliferating cells from limiting dilution assays were also bipotent. Combined in vitro and in vivo spheroid and matrigel assays revealed that these EPCs exhibit vasculogenic capacity by forming functional blood and lymph vessels. Conclusion The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell.en
dc.titleMouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vesselsen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderSchniedermann et al.en
dc.date.updated2015-09-04T08:28:40Zen
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