Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

2.50
Hdl Handle:
http://hdl.handle.net/10033/620767
Title:
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Authors:
Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula; Dragosits, Martin; Rodríguez-Carmona, Escarlata; Baumann, Kristin; Giuliani, Maria; Parrilli, Ermenegilda; Branduardi, Paola; Lang, Christine; Porro, Danilo; Ferrer, Pau; Luisa Tutino, Maria; Mattanovich, Diethard; Villaverde, Antonio
Abstract:
Abstract Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
Citation:
Microbial Cell Factories. 2008 Apr 04;7(1):11
Issue Date:
4-Apr-2008
URI:
http://dx.doi.org/10.1186/1475-2859-7-11; http://hdl.handle.net/10033/620767
Type:
Journal Article
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorGasser, Brigitteen
dc.contributor.authorSaloheimo, Markkuen
dc.contributor.authorRinas, Ursulaen
dc.contributor.authorDragosits, Martinen
dc.contributor.authorRodríguez-Carmona, Escarlataen
dc.contributor.authorBaumann, Kristinen
dc.contributor.authorGiuliani, Mariaen
dc.contributor.authorParrilli, Ermenegildaen
dc.contributor.authorBranduardi, Paolaen
dc.contributor.authorLang, Christineen
dc.contributor.authorPorro, Daniloen
dc.contributor.authorFerrer, Pauen
dc.contributor.authorLuisa Tutino, Mariaen
dc.contributor.authorMattanovich, Dietharden
dc.contributor.authorVillaverde, Antonioen
dc.date.accessioned2017-01-27T09:46:39Z-
dc.date.available2017-01-27T09:46:39Z-
dc.date.issued2008-04-04en
dc.identifier.citationMicrobial Cell Factories. 2008 Apr 04;7(1):11en
dc.identifier.urihttp://dx.doi.org/10.1186/1475-2859-7-11en
dc.identifier.urihttp://hdl.handle.net/10033/620767-
dc.description.abstractAbstract Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.en
dc.titleProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overviewen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderGasser et al.en
dc.date.updated2015-09-04T08:28:24Zen
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