Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions

2.50
Hdl Handle:
http://hdl.handle.net/10033/620773
Title:
Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions
Authors:
Garbe, Julia; Wesche, Andrea; Bunk, Boyke; Kazmierczak, Marlon; Selezska, Katherina; Rohde, Christine; Sikorski, Johannes; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Jahn, Dieter; Schobert, Max
Abstract:
Abstract Background Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. Phage therapy is an alternative to treat these chronic P. aeruginosa infections. However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages. Results We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. Sequencing of the phage genome revealed that the phage JG024 is highly related to the ubiquitous and conserved PB1-like phages. The receptor of phage JG024 was determined as lipopolysaccharide. We used an artificial sputum medium to study phage infection under conditions similar to a chronic lung infection. Alginate production was identified as a factor reducing phage infectivity. Conclusions Phage JG024 is a suitable broad host range phage which could be used in phage therapy. Phage infection experiments under simulated chronic lung infection conditions showed that alginate production reduces phage infection efficiency.
Citation:
BMC Microbiology. 2010 Nov 26;10(1):301
Issue Date:
26-Nov-2010
URI:
http://dx.doi.org/10.1186/1471-2180-10-301; http://hdl.handle.net/10033/620773
Type:
Journal Article
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorGarbe, Juliaen
dc.contributor.authorWesche, Andreaen
dc.contributor.authorBunk, Boykeen
dc.contributor.authorKazmierczak, Marlonen
dc.contributor.authorSelezska, Katherinaen
dc.contributor.authorRohde, Christineen
dc.contributor.authorSikorski, Johannesen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorJahn, Dieteren
dc.contributor.authorSchobert, Maxen
dc.date.accessioned2017-01-27T10:21:38Z-
dc.date.available2017-01-27T10:21:38Z-
dc.date.issued2010-11-26en
dc.identifier.citationBMC Microbiology. 2010 Nov 26;10(1):301en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-10-301en
dc.identifier.urihttp://hdl.handle.net/10033/620773-
dc.description.abstractAbstract Background Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. Phage therapy is an alternative to treat these chronic P. aeruginosa infections. However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages. Results We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. Sequencing of the phage genome revealed that the phage JG024 is highly related to the ubiquitous and conserved PB1-like phages. The receptor of phage JG024 was determined as lipopolysaccharide. We used an artificial sputum medium to study phage infection under conditions similar to a chronic lung infection. Alginate production was identified as a factor reducing phage infectivity. Conclusions Phage JG024 is a suitable broad host range phage which could be used in phage therapy. Phage infection experiments under simulated chronic lung infection conditions showed that alginate production reduces phage infection efficiency.en
dc.titleCharacterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditionsen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderGarbe et al.en
dc.date.updated2015-09-04T08:28:08Zen
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