2.50
Hdl Handle:
http://hdl.handle.net/10033/620776
Title:
Dengue-specific subviral nanoparticles: design, creation and characterization
Authors:
Khetarpal, Niyati; Poddar, Ankur; Nemani, Satish K; Dhar, Nisha; Patil, Aravind; Negi, Priyanka; Perween, Ashiya; Viswanathan, Ramaswamy; Lünsdorf, Heinrich; Tyagi, Poornima; Raut, Rajendra; Arora, Upasana; Jain, Swatantra K; Rinas, Ursula; Swaminathan, Sathyamangalam; Khanna, Navin
Abstract:
Abstract Background Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate. Methods We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally. Results The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence. Conclusions VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
Citation:
Journal of Nanobiotechnology. 2013 May 25;11(1):15
Issue Date:
25-May-2013
URI:
http://dx.doi.org/10.1186/1477-3155-11-15; http://hdl.handle.net/10033/620776
Type:
Journal Article
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorKhetarpal, Niyatien
dc.contributor.authorPoddar, Ankuren
dc.contributor.authorNemani, Satish Ken
dc.contributor.authorDhar, Nishaen
dc.contributor.authorPatil, Aravinden
dc.contributor.authorNegi, Priyankaen
dc.contributor.authorPerween, Ashiyaen
dc.contributor.authorViswanathan, Ramaswamyen
dc.contributor.authorLünsdorf, Heinrichen
dc.contributor.authorTyagi, Poornimaen
dc.contributor.authorRaut, Rajendraen
dc.contributor.authorArora, Upasanaen
dc.contributor.authorJain, Swatantra Ken
dc.contributor.authorRinas, Ursulaen
dc.contributor.authorSwaminathan, Sathyamangalamen
dc.contributor.authorKhanna, Navinen
dc.date.accessioned2017-01-27T10:30:39Z-
dc.date.available2017-01-27T10:30:39Z-
dc.date.issued2013-05-25en
dc.identifier.citationJournal of Nanobiotechnology. 2013 May 25;11(1):15en
dc.identifier.urihttp://dx.doi.org/10.1186/1477-3155-11-15en
dc.identifier.urihttp://hdl.handle.net/10033/620776-
dc.description.abstractAbstract Background Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate. Methods We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally. Results The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence. Conclusions VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.en
dc.titleDengue-specific subviral nanoparticles: design, creation and characterizationen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderKhetarpal et al.; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:27:52Zen
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